鸡传染性支气管炎抗体elisa诊断方法研究-传染病与预防兽医学专业论文.docxVIP

AbstractAvian Abstract Avian infect{ous bronchi{is i s an acute．highly touched infeetious(1isease caused by infectious bronchitis viFUN，which is one of the disease threaten poultry industry．Our study is to estabtish a convenient。specific and rapid deteetive technique for routine measuring antibodies to infeetious bronchiti8 virus．Method：IBV was a propagated in the altantoic cavity of 9--day—old specific pathogen free(SPF)embryes，as we]l as innulated in the monolayer ofchicken embryofibroblasts(CEF)by serial passage．#01lowed purification．then measuring prolein quantity，which is used coated antigen．Conjugation was to label the IIRP to rabbi t anti-chichen IgG using periodate method，TMB was as substrate，to set up enzyme—linked immunosorbent assay(ELISA) for testing antibodies to infectious bronchitis virus．Results：IBV obtained by culturing in the CEF monolayer COUid be used to the coating antigen．No crossing reaction was observed with antibodies to Newcastle disease virus(NDV)，Marek’s disease virus(MI)V)，infectious bursa]di sease virus(IBDV)，infectious laryngotracheitis virus(ILTV)，Avian influenza virus(AIV) Avian parainfluenza virus(APIV)， Reticuloendotheliosis virus(REV)， Infectious Coryza(IC)，Myeoplasma gallisepticum(^IG)，Mycoplasma synoviae(MS)．IBV antigen having a better group specificing could be reacted with antibodies to IBV Gray strain、M strain、 Conn strain、T strain、B strain、H strain、Ark99 strain、SEl7 strain individually．The coincidence of result was good by comparingwith the imported reagent of IB—ELISA produced by IDEXX，Conclusion：Our developed IB～ELISA detective reagent can be used to test antibodies to lBV Key words：infectious bronchitis virus，enzyme—Iinked immunosorbent assayr(ELISA) ant ibodies to{甜 独创性声明本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成 独创性声明 本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成 果。尽我所知，除了文中特别加以标注和致谢的地方外，论文巾不包含其他人已经发 表或撰写过的研究成果，也不包含为获得中国农业大学或其它教育机构的学位或证书 而使用过的材料。与我～同工作的同志对本研究所做的任何贡献均已在论文巾作了明 确的说明并表示了谢意。 研究生签名：妻11构 时问：3。oy年lf月，弘H 关于论文使用授