积雪草苷的提取与分离-生物化工专业论文.docxVIP

西安建筑科技 西安建筑科技大学硕士学位论文 西安 西安建筑科技大学硕士学位论文 Extract and Separation of Asiaticoside Speciality : Biochemical Engineering Name: Wenyan HOll 1nstructor: Renxiao Gao Associate Prof Abstract Centella asiatica is a kind of common traditional Chinese medicine collected in Chinese pharmacopoeia which belongs to Umbelliferae of Centella . lts able to clear heat，promote diuresis and remove toxic substances and promote subsidence of swelling by means of its cold，bitter，pungent feature. Asiaticoside and Madecassoside are active principle of Centella asiatica，which have function of antioxidation ，antibiosis， antidepressiv，immunomodulation etc. 1n order to make better use of Centella asiatica and research pha口nacology activity of every ingredient ，separation and making lots of Asiaticoside and Madecassoside with high purity are necessary lt is studied systematicallv on the craft condition of separation and extraction of Asiaticoside and Madecassoside from raw materia l. Through breaking，Skim ethanol-extraction silica column chromatography ，preparative chromatography etc The higher purity product for injection is got. Chemical method- Acid-hot and Alkali-hot method and mechanical method-Frozen-Thawing method are used to crush cellAfter observation of crush condition by electron m icroscope ，it is fOllnd that these methods can crush cells pm1ly ; especially，Acid-hot and Alkali -hot methods are more effective. Then ，taking cell rupturing as prem ise，condllct experiment with Asiaticoside and Madecassoside compared with tradit ional m ethod -- Ethanol thermal circllmtlllence method ，to find that these method s are more effective and the A lkali -hot method is the most effective. Ultrasonic method can make cavitation and also the bllbbles in solution which increase and make blasting compression ，then substance in cells disperse ， increasing contact area between substance inside cells and extracting solvent. So the mass transfer rate from plants cell to liquid p