麦麸膳食纤维与低聚糖的制备及理化性质研究-食品科学专业毕业论文.docxVIP

ABSTRACTThe ABSTRACT The technology of release soluble dietary fiber from wheat bran through alkali extraction and alcohol precipitation，preparation of oligosaccharides from wheat soluble dietary fiber by composite enzyme of xylanase and cellulose were investigated，and nature in t11iS research．The main results were as follows： The composition of wheat bran was analyzed and found to be as follows：moisture 12．10％，lipid 3．21％，protein 15．00％，starch 17．41％，ash 4．38％，SDF 0．95％，IDF 54．67％， TDF 55．72％，soluble pentosan 0．64％and insoluble pentosan 1 7．4％． The wheat bran starch was enzymatic hydrolyzed by 0【-amylase and glucoamylase． The optimal conditions for 0【-amylase hydrolysis were：temperature 95。C，hydrolysis time 1 5min，enzyme added 240U／g．The optimal conditions for glucoamylase hydrolysis were： temperature 60℃，hydrolysis time 30min，enzyme added 1 0000U／g． Using alkaline protease to hydrolyze and establishing its optimal conditions： temperature 60。C，hydrolysis time 30min，enzyme added 0．2AU／g． Wheat bran used as raw materials，the technology of alkali extraction were studied in order to prepare soluble dietary fiber．By the response surface method(RSM)test，the optimum technological parameters of preparation were determined on the basis of single factor experiments，the optimum conditions were：ratio of solid to liquid 1：2 1(w／v)，pH 1 1．75，temperature 85℃，time 2h and HE02 concentration O．45％．Under t11iS condition，the yied of soluble dietary fibre would be reached 1 1．45％． The wheat bran soluble dietary fibre was enzymatic hydrplyzed by xylanase and cellulose to produce oligosaccharides．The optimal conditions were：pH 5．5，temperature 55℃，xylanase added 600U／g，cellulose added 3 1 3U／g，hydrolysis time 2．5h． Study the differences of physicochemical properties and morphology on the soluble dietary fibre and oligosaccharides．Soluble dietary fibre after xylanase and cellulose degradation，SEM observation showed that soluble dietary fibre Was loose structure and sheet，the form of oligos