外界条件对冬虫夏草胞外多糖构象的影响.pdf

62 2012, Vol. 33, No. 13 食品科学 ※基础研究 1,2 2 3 闫景坤 ，李 琳 ，吴建勇 (1.江苏大学食品与生物工程学院，江苏 镇江 212013 ； 2.华南理工大学轻工与食品学院，广东 广州 510640 ；3.香港理工大学应用生物与化学科技学系，香港) 以人工培养冬虫夏草菌丝发酵液中分离纯化的胞外多糖EPS-1A 为原料，利用紫外光谱和圆二色谱研究不 同外界条件如处理温度、金属离子(Ca2+) 、变性剂(尿素)及化学修饰剂(刚果红)对胞外多糖 EPS-1A 构象的影响。结 果表明：刚果红反应揭示胞外多糖 EPS-1A 为无规线团链构象；外界条件均不能改变胞外多糖EPS-1A 在溶液中的 无规线团链构象，只是在不同程度上影响多糖分子的分子内和分子间氢键相互作用，使多糖变得松散、无序。 冬虫夏草；胞外多糖；构象；紫外光谱；圆二色谱 Influence of External Conditions on Conformation of Exopolysaccharide from Cordyceps sinensis 1,2 2 3 YAN Jing-kun ，LI Lin ，WU Jian-yong (1. College of Food Science and Biological Technology, Jiangsu University, Zhenjiang 212013, China； 2. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China； 3. Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hongkong, China) Abstract：In this study, ESP-1A, an exopolysaccharide isolated and purified from the fermentation broth of cultured Cordyceps sinensis fungus Cs-HK1 was used to study the effects of temperature, metal ion (Ca2+), denaturing agent (urea) and chemical modifier (congo red) on its confirmation by means of ultraviolet (UV) spectroscopy and circular dichroism (CD) spectroscopy. The results indicated that congo red reaction revealed that EPS-1A was a random coil chain conformation. External conditions could not alter the random coil chain conformation of EPS-1A in aqueous solutions, but influence the inter- and intra-molecular hydrogen bond interactions of polysaccharide molecules in some extent so that EPS-1A became m