马铃薯体细胞杂种的青枯病抗性鉴定-蔬菜学专业毕业论文.docxVIP

6．综合利用方面，体细胞杂种中CHT-3、CHT-5、CHT一6、CHT一7、CHT一9、CHT一10、 6．综合利用方面，体细胞杂种中CHT-3、CHT-5、CHT一6、CHT一7、CHT一9、CHT一10、 CHT一14、CHT一15、CHT一18属于抗性材料，是比较理想的抗青枯病材料，可在经过改 良后加以利用：而CHT一11、CHT—12、CHT一16、PHT～33属中抗材料，可在改良后加 以利用，其中前3个材料有望经过适应性种植，在观察其抗性及农艺性状的遗传稳 定性后，作为抗青枯病品种推广种植。 7．在抗病育种利用前景方面，马铃薯品种对青枯病的抗性具高度的小种特异 性，在一定程度上是对环境适应性的表现，尤其受温度的影响，其抗性在不同地区 会有不同的表现。有必要通过群体改良及回交的办法，提高体细胞融合材料的育性， 改良其野生性状，进一步扩大抗青枯病基因背景，提高数量性状遗传效率，同时提 高抗青枯病种质材料对其他病虫害的抗性及改善其熟性、适应性、丰产优质等优良 农艺性状。实验结果表明，体细胞融合技术与常规育种技术相结合，为利用马铃薯 家族中野生种的丰富优良抗性基因提供一种新的方法，为马铃薯栽培品种遗传改良 以获得优良的抗性基因和农艺性状提供了一条高效快捷的途径。 主题词：马铃薯；体细胞杂种；青枯病；抗性鉴定 ABSTRACTPotato(Solanum ABSTRACT Potato(Solanum tuberosum L．)is the fourth biggest food crop in both world and China and has very significant economic importance，Bacterial wilt(BW)，caused by Ralstonia solanacearum，is cosmopolitan，especially in WOrlTI regions like southwest China，where usually experiences about 20％loss of potato yield due to BW which is only the second to potato late blight． Under the conditions of Liupanshui of GuizhOU Province where BW epidemics frequently，a survey on distribution of the pathogen racies／biovars was carried out and evaluation of the resistance to bacterial wilt of the somatic hybrids．which were deftved in Huazhong Agricultural University from the protoplast fusion between dihaploid of s tuberosum 8 1—1 5 and diploid cultivated species S phureja and wild species S chacoense，was conducted in addition to the investigation of their morphological and biological characteristics aiming at to approach the capability of the somatic fusion strategy in transferring BW resistance between species and potential use of the somatic hybrids in potato breeding programme The main results gained include： 1．It was found from the experiment of inoculation with mixed races of R solanacearum in greenhouses that clones of somatic hybrids CHT-3 and CH1-5 performed high resistance to BW,Clones CHT-1l，CHT-12，CHT．16，PHT-17，PHT-24 and PHT-33 had the mid—level resistance．Clone CHT．4 was susceptible to BW w