马铃薯花青素转录激活基因的克隆与序列分析-作物遗传育种专业毕业论文.docxVIP

Cloning Cloning and Sequence Analysis of Anthocyanin 11ranscriptional Activator Genes of Potato Abstract Anthocyanin has some important physiological functions，such as antioxidation， antimutation，antiproliferation etc．Purple potato is intituled healthy food for its abundant anthocyanill Pigmentation pattems and the intensity of anthocyanin in Various plallt tissues are coordinately comr011ed by me expression ofanthocyanin transcriptional actiVator genes in plants．Clonin譬and identiflcation of anthocyanin transcriptional activator genes of potato(MYC and MYB like genes)are Very usefhl for elucidation t11e mechallisms t11at controlthe patial aIld temporal pigmentation in potato and for controlling the pigmentation in potato by using the anthocyanin transcriptional actiVator genes as switches． In this study，the conserVed 5—七nd fragment(Stmycl)of myc like an血ocyanin traIlscriptional activator gene、vas唧plmed by using爵卜PCR丘om the RNA of purple potato coat，Then the cDNA 3-end sequence(stmyc2)was amplified by using 3’一Race technique from the RNA of purpIe potato coat．The myc like anthocyanin tra芏1scriptional activator gene(Stnlyc)of potato was got by connecting Stmycl fragment afld Stmyc2 fragment．The lengt王l of Stmyc gene is 1 1 06bp，containing an intact ORF and coding 326aa At the s锄e time，the conse“ed fragment(Stmybl)of myb like aHmocyanin tmscriptional activator gene was锄plinedby using R卜PCR from me RNA of purple potato coat．They belong to the R2R3 myb conserved fragment(named Stmyb280 and Stmyb2 11)of myb like anthocyanin transcriptional actiVator gene． Conclusion： 1)we is01ated the myc like aIlmocyanin transcriptional activator cDNA(Stmyc)from purple potato coat．The length of it is 1106bp，Which contained an intact ORF The ORF located between positions 1 O a11d 990 aI】d coding 326aa．After sequence analysis，The deducedprotein ofstmyc geneis similartothat ofPP，ff肠少“据占cPnJ，the similarityis 94％ Stmyc gene encodes a bHLH protein domain that is more imponant for MYC—l