毛白杨瘿木形成过程中结构、化学组成及细胞内含物含量变化的研究-森林培育专业毕业论文.docxVIP

摘要本文以一年生毛白杨为实验材料，从接种根癌农杆菌后形成的毛白杨瘿瘤和树体 摘要 本文以一年生毛白杨为实验材料，从接种根癌农杆菌后形成的毛白杨瘿瘤和树体 的构造、木材化学组成、细胞内含物含量方面研究，旨在探讨瘿木形成机制，主要结 果如下： 显微构造分析表明，接种根癌农杆菌后，毛白杨接种部位两侧细胞增生，细胞增 生较快的部位形成放射式导管。其瘿瘤是由于接种部位两侧大量细胞增生，将寄主的 部分茎结构挤向外侧而形成的，瘿瘤表面可以重新形成木质化结构。瘿瘤丰富的维管 束直接与寄主维管束系统相连。其中的导管呈树枝状分支和涡旋状。在接种部位之下 茎组织分化出较宽的髓射线，导管直径显著变大；接种部位之上导管直径明显减小。 解剖构造分析结果表明：纤维各解剖参数(壁厚除外)在处理株接种部位明显下 降，瘿瘤上方木质部和接种部位导管变小：下方受其影响较小。对照株不同接种部位 木质部纤维和导管无明显变化。毛白杨处理株接种部位形成的瘿瘤之『白J纤维及导管分 子的长、宽均差异显著(5％水平)，说明瘿瘤细胞分裂异常。与毛白杨木质部相比， 瘿瘤中导管和纤维明显减小。 化学组成分析结果表明，处理株接种处冷水抽出物、热水抽出物、1％NaOH抽 出物、木质素含量增加，纤维素含量降低。瘿瘤中冷水抽出物、热水抽H{物、1％NaOH 抽出物含量分别比木质部提高了2．64．3倍、1．5~2，6倍、1．1一】．8倍，而纤维素含量 下降了5l％r99％。处理株接种部位木质素是瘿瘤的2．7倍。和其它接种部位相比， 瘿瘤中木质素含量略高。 细胞内含物含量变化分析结果表明：和对照株相比，处理株根系和叶片中的可溶 性糖、蛋白质、K、Ca含量均降低，而处理接种处木质部和韧皮部中内含物含量明显 升高。毛白杨瘿瘤在生长过程中内含物含量发生变化，但总的趋势是增加的。由此我 们可以认为营养优先供应给接种处和瘿瘤致使其它部位含量降低。 关键N．-毛白杨；瘿木；木材构造；木材化学组成；细胞内含物 Study Study On structure characteristics，chemical compositions and cell inclusion contents of burl during its development in Chinese white poplar Author：Houxicheng Major：Forestry cultivation Supervisor：Yuanyuxin Dukeiiu Abstract 1nhe structure characteristi铬．chemical compositions and eell inclnsion contents of hurl development mechanism，with induced by Agrobacterium tumefaciens，were tested on !-year old Chinese white poplar seedling．The results obtained ate showed as follows： With the micro-structure analysis，it demonstrates that the position induced by A． tumefaciens would make cell hyperplasia at the beginning around the wound，of which it established vessel differentiation at the faSt developing part．The burl tissue is forming slowly with those of cell hyperplasia that extrudes some of host stem tissue to develop all apophysis on the skin of stem of Chinese white poplaL in which it would established new structure—lignified．There are upgrowth vascular bundle that conuect with host’S vaScular system in well·developed burl，of which its present shapes of tree-like and swirl．At the same time，the more width rays are developing and the vessels diameter are in