黄连、生地及其配伍对2型糖尿病大鼠治疗作用及机制的比较研究-中西医结合临床专业毕业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 5 PAGE 5 Comparative study on therapeutic effect and its mechanism of coptidis rhizome, rehmannia dride rhizome and their compatibility on type 2 diabetic mellitus rats Candidate : Zhao Jing Supervisor : Yang Mingwei Abstract Objective To observe the effects of coptidis rhizome, rehmannia dride rhizome and their compatibility on insulin resistance （IR）and function of islet beta cell in type 2 diabetes mellitus rats so as to investigate mechanisms of coptidis rhizome, rehmannia dride rhizome and their compatibility, and explore their compatibility whether there are selective or synergistic effects on therapeutic targets and reveal further the scientific connotation of Rhizoma Coptidis Pill. Method The mode of type 2 diabete mellitus rats was induced by high fat fed and low dose Streptozotocin (STZ) (30mg/kg) injected through vena caudails. The diabetic rats were randomly divided into the following five groups: coptidis rhizome group, rehmannia dride rhizome group, coptidis rhizome + rehmannia dride rhizome group, rosiglitazone group and diabetic control group. Normal control group was also set up. Afer intervention for four weeks, oral glucose tolerance test (OGTT) and insulin release tests (IRT) were done on each group. The blood of each rat was collected via ophthalmic venous plexus at 0, 30, 60,120 and 180 min afer rats were anesthetized by the ether. After IRT finished, all rats were anesthetized. Blood was collected from aorta ventrail.Pancreas were immediately also dissected out. A portion of pancreatic tissues were put into 10% neutro-formalin solution for histopathological study and islet beta cells apopotosis detection. The other portions of pancreatic tissues were put into 2% glutaric dialdehyde for observation of islet beta cell ultrastructure. Blood glucose levels were tested by the means of gluocose oxidation enzyme. Serum insulin was tested by the means of radio-immunity. Serum IL-1β, IL-6 and TNF-α were teste