慢性间歇性低氧和高脂饲养对兔胰岛β细胞功能的影响研究-老年医学专业毕业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 8周时达最高水平(P0．05)。FINS组间及组内两两比较差异无显著性 意义。 3．2x2析因分析显示CIH对13细胞功能各指标无显著影响， HOMA-p、FINS／FBG、AUCills(o-I岫)和AIR的显著性概率P分别为0．78、 0．80、0．17、0．23；高脂饲养对13细胞功能及第一时相分泌受损有影响， HOMA-IB、FINS／FBG、AUC积o-lo幽)和AIR的显著性概率P分别为0．001、 O．02、0．03、O．04；慢性间歇性低氧与高脂饲养对13细胞功能4个指标 不存在交互作用，显著性概率P分别为0．56、0．60、0．39、0．35。 结论1．高脂饲养可使新西兰兔机体胰岛B细胞功能下降，第一时相 分泌受损； 2．观察并评价了慢性间歇性低氧及其与高脂饲养的联合作用 对新西兰兔胰岛13细胞功能的影响。nOMa-IB、FINS／FBG、J址坨i。蚺l岫) 及AIR4种胰岛B细胞功能指标未显示慢性间歇性低氧及其与高脂饲养 的联合作用明显损害胰岛D细胞功能及第一时相分泌。 关键词阻塞性睡眠呼吸暂停低通气综合征，慢性间歇性低氧，高 脂饲养，胰岛p细胞功能 Ⅱ 硕士学位论文 硕士学位论文 英文摘要 Abstract objective To investigate the effects of c11romc intermittent hypoxia (Cm)and high-fat-feeding on the islet p cell function,and analyse the synergistic effect between CIH and high-fat-feeding．To present a new view for the pathologic association between OSAHS and T2DM． Methods To啪prospective animal experiment—andomized controlled study and factorial experiment．The 24 New-Zealand white rabbits(4 months olm were randomly and equity devided into 4 groups, which were control group(C)，c如omc intermittent hypo)【ia group(H)，high- fat-feeding group④and hypoxiahigh-fat-feeding group qH-F)．Every group involved 6 rabbits．Group H and H+F were placed in an environment into which nitrogen and air were periodically infused every 8 minutes for 5h／day．The loweg level of oxygen concentration was at 8％．Group F and H+F were feeded with fat-rich-stoyer．The intervention experiment lasted for 8 weeks．Blood samples for me舢emenm of total cholesterol(CH)，low density lipoprotein cholesterol(LDL-CH)and free fatty acids(FFA)，fasting blood glucose(FBG)and fasting insulinffINS)were withdrawn at O,4W and 8W．Intravenous glucose tolerance tests(IVGrr)were performed at the end of the interventions．Blood samples for measureme