麻黄碱对人支气管上皮细胞增殖及表达Eotaxin和IL-8的影响内科学专业毕业论文.docxVIP

PAGE PAGE 10 组比较，C 组 Eotaxin 浓度明显升高，差异有统计学意义(P0.01)。与 C 组比较，D、E、F 组 Eotaxin 浓度均明显降低，F 组最低，差异均有 统计学意义(P 均0.01)；A 至 F 组细胞上清液中 IL-8 浓度(pg/mL)分 别为 17.45±2.32，18.78± 1.05，27.24± 1.91，23.62±3.40，19.25±2.36， 14.12 ±3.14，各组间比较差异有统计学意义(P0.01)。与 A 组比较，C 组 IL-8 浓度明显升高，差异有统计学意义（P0.01）。与 C 组比较，D、 E、F 组 IL-8 浓度均明显降低，F 组最低，差异均有统计学意义（P 分 别0.05，0.01，0.01）。 结论：⑴在一定浓度范围内，麻黄碱对体 外培养的 16HBE 形态及增殖无影响；⑵麻黄碱能抑制前炎症因子 TNF-α 刺激 16HBE 表达及分泌 Eotaxin 和 IL-8，这可能是中药麻黄治 疗哮喘的机制之一；⑶麻黄碱与糖皮质激素抑制 16HBE 表达及分泌 Eotaxin 和 IL-8 具有协同作用。 素-8 关键词：麻黄碱；气道上皮细胞；嗜酸性粒细胞趋化因子；白介 Effects of ephedrine on proliferation and expression of Eotaxin and IL-8 in human bronchial epithelial cells Abstract Objective: To study the effect of ephedrine on morphology, proliferation and expression of Eotaxin and IL-8 in human bronchial epithelial cells (16HBE) cultured in vitro, and to explore the mechanism of ephedra in the treatment of asthma. Methods: The 16HBE strain were subcultured in cell culture flask and the fourth or fifth generation cells in good state were choosed for the experiment: (1) The 16HBE were seeded in 6-well plates and 96-well plates, and randomly divided into five groups, and then interfered sequentially with ephedrine at different concentration (0, 150, 300, 600, 1200 μg/mL ) for 24, 48, 72 hours. The morphological and growth status of 16HBE were observed by inverted phase contrast microscope. The cell proliferation was detected by CCK-8 assay . (2) The 16HBE were inoculated in 6-well plates and randomly divided into six groups: ① Control group (group A): 16HBE were incubated in complete medium for 18h; ② Ephedrine group (group B): 16HBE were incubated in complete medium containing 300 μg/mL ephedrine for 18h; ③ TNF-α group (group C): 16HBE were incubated in complete medium containing 20ng/mL TNF-α for 18h; ④ Ephedrine plus TNF-α group (group D): 16HBE were pre-incubated with 300 μg/mL ephedrine for 1 hour before being added to 100 ng/mL TNF-α for 18h; ⑤ Dexamethasone plus TNF-α group (group E): 16H