滤蓝光人工晶体对猪视网膜色素上皮细胞保护作用-眼科学专业毕业论文.docxVIP

渐讧大学硕}母f亢生学位论文blue-light 渐讧大学硕}母f亢生学位论文 blue-light absorbing intraocular lens and pig retinal pigment epithelium protection in vitro Post-graduate： Zhu li-li Teacher ：Lou ding-hua [Abstract]0bjecfive To study the effect of blue-light absorbing intraocular lens to protect retinal pigment epithelium(RPE)from blue light damage． Methods The pig retinal pigment epithelium are primary cultured and are used for experiment during from third to fifth degeneration．The subjects included：cells proteced by blue—light absorbing lens， proteced by common lens，directly exposured to blue light and unexposured to blue l ight．All the cultured pig retinal pigment epithelium were illuminated with blue light(50000+10000lux)．Comet assay was used to detect the DNA damage of retinal pigment epithelium． Results：There Was a significant difference between cells with blue light direct irradiation and without it for MTL and MTM fP0．01)．There wasn’t significant difference between cells with normal intraocular lens and no intraocular lens for MTL and MTM(P0．05)．There was a significant difference between cells with blue—light absorbing lens and normal intraocular lens for MTL(PO．05)．And there Was a significant difference between cells with blue-light absorbing lens and ceils with 浙江大学硕：t研究生学位论文blue 浙江大学硕：t研究生学位论文 blue light direct irradiation(PO．05)． Conclusions The resuls tentatively confirmed that blue light could induce pig retinal pigment epithelium cellular indury,and blue—light absorbing lens protect pig retinal pigment epithelium from blue light damage to cells． [Key words】blue—light absorbing lens；retinal pigment epithelium； blue-light；DNA damage；comet assay 研究正文视网膜色素上皮细胞位于视网膜的最外层，具有重要的生理功能。一 研究正文 视网膜色素上皮细胞位于视网膜的最外层，具有重要的生理功能。一 旦视网膜色素上皮细胞发生功能障碍将会发生年龄相关性黄斑变性， 视网膜色素变性等一系列的眼部疾病。近年来的大量实验室证据表明 位于可见光边缘的蓝光可以造成视网膜色素上皮细胞的损伤n一1，其 中光照激活作用首先造成DNA损伤“1。而彗星实验作为一种检测单细 胞DNA损伤的方法灵敏性高(可检测到每0．9道尔顿0．IDNA损伤)， 所需细胞样本量少(≥1000个)、‘快速、简便、重复性好。为此，本 试验采用彗星实验研究滤蓝光人工晶体对视网膜色素上皮细胞所受 的蓝光损伤是否具有防护作用。 1．材料与方法 1．1材料 培养的