慢性阻塞性肺疾病大鼠模型细胞因子水平变化的分析-内科学(呼吸系病)专业毕业论文.docxVIP

万方数据 万方数据 FEV0.3/FVC 升高（P 0.01 或 P 0.05）;pH、PaO2 升高，PaCO2 降低;病理改变 减轻;血清中 IL-8、TNF-α、MMP-9 的浓度有所降低，而 IL-10 浓度升高;肺组织 中 IL-8、TNF-α、MMP-9 阳性表达率降低（P 0.01 或 P 0.05）、IL-10 阳性表 达率升高，以治疗 6 周变化明显。结论：1.采用气管内两次注入 LPS 及熏香烟的 复合刺激法可成功建立 COPD 大鼠模型，该模型符合人类 COPD 的病理特点。 2.COPD 模型大鼠随着造模时间的延长，血清和肺组织 IL-8、TNF-α、MMP-9 表 达随之升高，IL-10 表达随之降低，在疾病早期就有明显变化；提示这些因子与 疾病的发生发展有关，监测这些因子有助于评诂 COPD 病情程度。3.经氨茶碱治 疗后，IL-8、TNF-α、MMP-9 表达水平下降，IL-10 表达升高，说明对这些因子 的监测也有助于对 COPD 治疗效果的评价。 【关键词】慢性阻塞性肺疾病；大鼠；细胞因子；肺功能；动脉血气 III Study on the changes of cytokine levels in a rat model of chronic obstructive pulmonary disease Major: Pharmacology Postgraduate: CHEN Yan Supervisor: LI Ling [Abstract] Objective:To establish chronic obstructive pulmonary disease(COPD) rat models, and monitoring the cytokine levels in rat models with COPD indifferent periods and the therapeutic action of aminophylline on COPD, to explore assessment value of cytokine levels on chronic obstructive pulmonary disease. Methods: COPD rat models was established by using two injection of LPS and the complex stimulation of smoked cigarettes. Continue building for 8 weeks. Rat general situation in the process of building were observed .The 0.3s forced expiratory volume(FEV0.3),the ratio of 0.3s forced expiratory volume and forced vital capacity ratio(FEV0.3/FVC) and arterial blood gas were determined after the end of the administration four weeks, six weeks and eight weeks; then pathomorphology of models’ lung were investigated by HE staining and Enzyme-linked immunosorbent assay was used to measure the concentrations of interleukin-8(IL-8),interleukin-10 (IL-10),tumor necrosis factor alpha(TNF-α) and matrix Metalloproteinase-9(MMP-9) in serum; the expressions of IL-8,IL-10,TNF-α and MMP-9 in tracheal lung tissues of rats were detected by immunohistochemistry. Stop passive smoking in rats after eight weeks, and aminophylline treatment, the treatment process general of COPD model rats were observed. And these respectively indicators were detected in