泌尿生殖道感染沙眼衣原体多态膜蛋白F基因的多态性研究-病原生物学专业毕业论文.docxVIP

万方数据 万方数据 目 录 中文摘要··············································································1 英文摘要·················································································4 研究论文 泌尿生殖道感染沙眼衣原体多态膜蛋白 F 基因的多态性研究 前言·················································································8 材料与方法·································································10 结果···············································································15 附图···············································································18 附表·····································································23 讨论·············································································24 结论·········································································26 参考文献·····································································27 综述 沙眼衣原体多态膜蛋白的研究进展·····································29 致谢····················································································41 个人简历·············································································42 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 泌尿生殖道感染沙眼衣原体多态膜蛋白 F 基因的多态性研究 摘 要 目的：泌尿生殖道沙眼衣原体的感染是世界范围内最常见的性传播疾 病之一，其感染率已经超过淋病奈瑟菌，而跃居性传播疾病之首，在我国 也有逐年上涨的趋势。与筛查或抗生素治疗相比，疫苗接种更经济，并对 全球控制沙眼衣原体感染有更大的影响，因此普遍认为疫苗接种是降低沙 眼衣原体(Chlamydia trachomatis,C.t)感染率的最好办法。目前，虽有大量 关于衣原体的疫苗研究，但至今尚无成熟疫苗问世。目前亚单位疫苗及 DNA 疫苗的研究焦点是找到合适的抗原，现在研究比较多的抗原有主要 外膜蛋白（Major Outer Membrane Protein，MOMP)，衣原体蛋白酶样活性 因子（Chlamydial Protease-Like Activity Factor，CPAF）等，但这些疫苗 均未达到理想的免疫效果。多态膜蛋白(Polymorphic Membrane Proteins， Pmp)是近年来发现的位于衣原体外膜上的一类蛋白，分子量比较大，而 且具有型特异性，保守性好。同时研究发现，Pmp 蛋白可能在 C.t 感染后 粘附宿主细胞和机体免疫应答中具有重要功能，为研制疫苗的潜在抗原。 因此本研究的目的是探讨泌尿生殖道感染患者沙眼衣原体多态膜蛋白 F （polymorphic Membrane Proteins F，PmpF）基因的多态性,为研究多态膜 蛋白 F 作为沙眼衣原体候选疫苗提供理论依据。 方法：收集 2013 年 1 月~2014 年 12 月某院泌尿科、妇科和皮肤科门 诊泌尿生殖道感染患者送检的分泌物标本，并对患者的性别、年龄、标本 来源进行详细登记。先用沙眼衣原体核酸诊断试剂盒进行初筛，将获得的 沙眼衣原体阳性标本采用巢氏 PCR 扩增 PmpF 基因，然后将扩增得到的 PmpF 基因用 Alu I、Hae III、Rsa I、Mob I 四种内切酶进行酶切，酶切图 谱采用 Gelcompar II (v