慢性高原病患者骨髓组织Bcl-2和Bcl-XL蛋白表达研究-内科学(血液病学)专业毕业论文.docxVIP

青海大学 青海大学 2009 级高校教师在职攻读硕士学位论文 第 第 PAGE 3 页 共 35 页 Abstract Objective In the plateau region, chronic mountain sickness (CMS) is a common disease, is characterized by over-accumulation of red blood cells and serious hypoxemia. Its pathogenesis is complex. Studies confirmed that excessive proliferation of erythroblasts played an important role. However, the study on the apoptosis of hematopoietic cells is limited. Recent studies showed that the apoptosis of bone marrow hematopoietic cells is downregulated in CMS. Lots researches proved that Bcl-2 and Bcl-XL proteins of Bcl-2 family distributed widely on surface of hematopoietic stem cells and progenitors, and are key factors in determining the apoptosis change. The aim of this study is to observe the expressions of Bcl-2 and Bcl-XL in bone marrow tissue of patients with CMS, exploring their possible roles in the developing of CMS. Methods The CMS was diagnosed according to “Qinghai diagnostic criteria for chronic mountain sickness” (VI World Congress on Mountain Medicine and High Altitude Physiology). The bone marrow specimens were collected from 20 patients with CMS (CMS group) and 18 patients with simple orthopedics (Control group). They were all males. The average ages were 39.7±9.4 years old in CMS group and 40.5±10.8 years old in Control group. The expressions of Bcl-2 and Bcl-XL in bone marrow tissues were detected using the immunohistochemistry technique. These parameters mentioned above were compared between CMS group and Control group, and the correlations among Bcl-2, Bcl-XL proteins and hemoglobin (Hb) concentration were analyzed. Results The positive rates of Bcl-2 and Bcl-XL proteins in CMS patients were 75.00% and 85.00%, respectively, while were 27.78% and 33.33% in control group, respectively. In the positive rates of Bcl-2 and Bcl-XL between these two groups, There were significant differences (P＜0.01). The positive coefficients of Bcl-2 and Bcl-XL proteins in CMS group were significantly higher