罗格列酮对急性坏死性胰腺炎及其肺损伤保护作用的实验分析-消化内科学专业毕业论文.docxVIP

英文摘要罗格列酮对急性坏死性胰腺炎及其肺损伤保护作用的实验研究 英文摘要 罗格列酮对急性坏死性胰腺炎及其肺损伤保护作用的实验研究 罗格列酮对急性坏死性胰腺炎及其肺损伤保护作用的实验研究英文摘要 罗格列酮对急性坏死性胰腺炎及其肺损伤保护作用的实验研究 英文摘要 IV IV PAGE PAGE V Experimental study of the protective effects of rosiglitazone on acute necrotic pancreatitis （ANP）and ANP-associated lung injury Abstract Part one Experimental study of the protective effects of rosiglitazone on acute necrotic pancreatitis Objective To investigate the protective effects and mechanisms of Rosiglitazone on acute necrotic pancreatitis (ANP). Methods seventy-two SD rats were randomized into three groups: sham operation (SO)group, ANP group and rosiglitazone -pretreated group. The model of ANP was induced by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct in SD rats, rosiglitazone -pretreated group were given 10mg/kg rosiglitazone intraperitoneally 30 min before inducing ANP. Rats in the three groups were killed at 3,6,12 hours after induction of the model. The levels of amylase in plasm, the myeloperoxidase(MPO) in the pancreas were measured. The levels of plasm TNF-α,IL-6 were detected by enzyme linked immunosorbent assay （ELISA）.The expressions of NF-κB in pancreatic tissue were assayed by immunohistochemistry,the expressions of PPARγmRNA and HSP-70mRNA in pancreatic tissue was detected by reverse transcript PCR（RT-PCR）.The histopathological changes and the histologic scores of pancreatic tissue were evaluated. Results （ 1 ） Compared with SO group, the levels of plasm amylase,TNF-α,IL-6, and the intrapancreatic MPO significantly increased in ANP group （ P0.05 ） , the expression of NF- κ B in pancreatic tissue significantly increased in ANP group（P0.01）, the histologic scores of pancreatic tissue significantly increased in ANP group （ P0.01 ） ,the pancreas injury were aggravated gradually.（2）Compared with ANP group, the levels of plasm amylase,TNF-α, IL-6 ,intrapancreatic MPO, and the expression of NF- κ B in pancreatic ti