毛细管电泳技术在玉米代谢组学中的应用研究-工业催化材料表征技术专业毕业论文.docxVIP

IV IV Abstract Capillary electrophoresis (CE) has been applied in many fields owing to its advantages of high separation efficiency and fast speed. However, the detection sensitivity of CE is greatly limited due to the small injection volume and the short optical path length. Currently, in order to improve the detection sensitivity of CE, various sample concentration methods have been investigated and developed. The metabolites of corns were analyzed by CE coupled with on-line concentration methods. The major content is described as follows: Two on-line concentration methods, namely field-enhanced sample stacking and dynamic pH junction, were utilized to improve the detection sensitivity. When field-enhanced sample stacking method was used, nine water-soluble vitamins were focused and separated baseline. When dynamic pH junction method was used, only nicotinic acid and folic acid were focused. The limits of detection of the vitamins were from 6 to 119 ng/mL (2.7×10-8 to 53.4 ×10-8 M) based on the signal-to-noise ratio of 3 and the relative standard deviations of migration time and peak area for each vitamin (1 μg/mL) were less than 3.5% using the field-enhanced sample stacking as an on-line concentration method. The developed method was applied to the analysis of water-soluble vitamins in corns and two vitamins, pyridoxine hydrochloride and nicotinic acid, were detected. The estimated concentration of them were 2.5 ± 0.11 μg/g - 6.67 ± 0.17 μg/g and 0.25 ± 0.01 μg/g - 0.67 ± 0.08 μg/g respectively. The recoveries of them were 87 %-106 % and RSDs were less than 6.81%. Two on-line concentration methods, namely field-enhanced sample stacking and dynamic pH junction, were utilized to improve the detection sensitivity. Seven purine bases and pyrimidine bases were focused and separated baseline using two methods. Comparing to field-enhanced sample stacking, dynamic pH junction was more suitable for the concentration of purine bases and pyrimidine bases since the sensitivi