慢速冷冻不同方法复温兔断肢再植后骨骼肌病理变化的研究骨科学专业毕业论文.docxVIP

山西医科大学硕士学位论文 山西医科大学硕士学位论文 II II 英文摘要 Study skeletal muscle of pathological changes of replanted rabbit extremities after slow-freezing in different of thawing methods ABSTRACT OBJECTIVE: Through the experimental study of cryopreservation of rabbit skeletal muscle tissue pathology changes after limb replantation, To provide feasibility and preliminary theory basis for replanting extremities after deep cryogenic treatment. METHODS: Take only 30 healthy New Zealand white rabbit, were divided into experimental group A and group B(A is slow-freezing and slow-thawinging ,B is slow-freezing and slow-thawinging ) and control group.The control group was made directly samples after amputated limb. Experimental group in dimethyl sulfoxide injection, heparin saline water elution, after dealing with the slow freezing, the slow thawing of group A, group B fast thawing after replantation and reattach blood after 6 hours away from the amputated limbs, collect A specimen of skeletal muscle made with the control group. RESULTS: General observation: after the experiment through blood group A muscle is ruddy color, good texture, elastic, stimulating contraction. Experimental group B: through blood after muscle swelling significantly, texture is poor, weak elastic, stimulate no obvious shrinkage. HE stained pathological histology changes: the control group: normal muscle cell and the nucleus form, without the muscle fiber fracture, did not see obvious cavity formation; Experimental group A: mild edema, muscle cells, part of the muscle fiber fracture, but the muscle cells than normal form, A small amount of inflammatory cell infiltration. Experimental group B: muscle cells, edema, muscle cells vacuolation in great quantities, a lot of muscle fiber fracture, there is a lot of inflammatory cells infiltration. Control group: sarcomere neatly, Z line clear, mitochondrial PAGE PAGE IV cristae density, cell membrane integrity, no swelling, structure is normal. Experim