毛细管电泳体内药物分析方法研究及应用-生物医学工程专业毕业论文.docxVIP

PAGE PAGE IV III III 体系的性能进行了考察，并用该法测定了贝诺酯在兔血中的代谢物——对乙酰氨 基酚的含量，获得了对乙酰氨基酚的药-时曲线，对乙酰氨基酚的血药峰浓度为 ×10-3 g·L-1，达峰时间是 4 h。 6．采用高效液相色谱法研究了麻黄碱在家兔体内的药代动力学过程。获得了 家兔单剂量注射麻黄碱注射液的动力学参数，消除速率常数为 0.299 h-1，生物半 衰期为 2.32 h。 关键词：体内药物分析，毛细管电泳，透析，化学发光 ABSTRACT Biopharmaceutical analysis in one of subjects which study pharmic relationships and varieties between quantity and quality in organism using kind of analytical methods in order to discover pharmacokinetic parameters, metabolite modes and routes etc. The object of biopharmaceutical analysis could be any of body fluides, organes, tissues or egestas. In all of these objects, blood and pharmaceuticals in it are the most commonly researched. Whereas, it is difficult to study biopharmaceuticals for their low concentration and many interferes from endogenous ingredients. So, fussy pretreatments are commonly needed and sensitive detection are desired. In this paper, a simple in-line dialysis capillary electrophoresis (CE) method is established to eliminate the interferes from endogenous macromolecules and simplify pretreatment. Moreover, the sensitive chemiluminescence and electrochemical detections for capillary electrophoresis are also established. The characteristics of the methods are evaluated. Additionally, some applications of the the methods in biopharmaceutical analysis are realized. The main research and results are as below: A novel CE method with dialysis sampling function was established. The dialysis membrane is in situ prepared in the inlet end of the separation capillary by phase-inversion process, which endued the capillary with an additional function of clean-up sampling. The materials and crafts of membrane preparation are researched. The ability of membrane cutting off macromolecules and particles is also assayed qualitatively. The membrane prepared using membrane-casting solution with components of 22% w/v polysulfone and 8% w/v PEG 600 can effectively cut off bovine serum albumin ( MW 18 kDa), while the membrane pr