日本晚樱花挥发油化学成分GC-MS分析及其抗氧化活性分析.PDFVIP

190 2013, Vol.34, No.20 食品科学 ※分析检测 日本晚樱花挥发油化学成分GC-MS分析及其 抗氧化活性分析 文飞龙，张璐璐，刘智慧，查钱慧，陈 杭，毕淑峰* (黄山学院生命与环境科学学院，安徽 黄山  245041) 摘  要：对日本晚樱花挥发油的化学成分进行气相色谱-质谱(GC-MS)分析，并以DPPH 自由基、ABTS＋ ·、亚硝酸钠清 除作用为指标，以1mg/mL VC或2,6-二叔丁基-4- 甲基苯酚(BHT)为阳性对照评价挥发油的抗氧化活性。结果表明：从挥发 油中鉴定出28个化合物，占挥发油总量的84.54%，以倍半萜和醛类为主；挥发油对DPPH 自由基、ABTS＋ ·、亚硝酸钠 有明显的清除作用，样品量与清除率间呈量效关系，且清除效果优于1mg/mL VC或BHT。 关键词：日本晚樱花；挥发油；气相色谱-质谱；抗氧化活性 Chemical Composition of Volatile Oil from Flowers of Prunus lanneciana Analyzed by GC-MS and Its Antioxidant Activity WEN Fei-long ，ZHANG Lu-lu ，LIU Zhi-hui ，ZHA Qian-hui ，CHEN Hang，BI Shu-feng* (College of Life and Environment Science, Huangshan University, Huangshan 245041, China) Abstract ：The chemical components of volatile oil from the flowers of Prunus lanneciana were determined by gas chromatography-mass spectrometry (GC-MS). The antioxidant activity of the volatile oil was evaluated by DPPH and ABTS free radical and sodium nitrite scavenging assays with 1 mg/mL VC or BHT as the positive control. The results showed that twenty-eight compounds representing 84.54% of the total constituents were identified. Some sesquiter- penoids and aldehydes were identified as the major components in the volatile oil. It scavenged DPPH and ABTS free radicals and sodium nitrite in a concentration-dependent manner, and showed better scavenging capacity than 1 mg/mL VC or BHT. Key words ：flowers of Prunus lanneciana ；volatile oil；gas chromatography-mass spectrometry (GC-MS)；antioxidant activity 中图分类号：TS201.2 文献标志码：A 文章编号：1002-6630(2013)20-0190-04 doi:10.7506/spkx1002-6630-201320040 -2-