胎肝干细胞治疗不同类型F344大鼠急性肝衰竭的实验研究及大鼠门静脉分支残端置管模型构建.doc

第四军医大学硕士学位论文 模型具有可反复、可选时、减少创伤等优点。 关键词：急性肝功能衰竭；干细胞移植；胚胎干细胞；动物模型；门静脉置管； -6- 第四军医大学硕士学位论文 a Study of the FLSPCs Transplantation effectiveness on different acute hepatic failure types of F344 mice And a Model of Acute Hepatic Failure with catheterization of portal venous branch Candidate for master:Guangxin Liu Supervisor:Kaishan Tao Department of Hepatobiliary Surgery of Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China Sponsored Programs: The National Natural Science Foundation of China 81170419) Abstract The acute liver failure is one of the main death causes of patients with certain liver diseases. The disease features symptoms including hepatic encephalopathy and fatal liver damage. The liver transplantation is considered to be the most effective treatment. The survival rate of transplantation is from 50% to 70%. Since the donor for liver transplantation is limited, more and more attentions are paid to the possibility of cell transplantation. Fetal liver stem/progenitor cells transplantation is one of the ideal methods. FLSPCs are obtained from embryonic-day (ED)-14 rat fetuses then enriched and purified by a three-step separation procedure previously reported by our group. To trace the exact effect of FLSPCs on amending liver injury, by liposome-mediated transfection, the green fluorescent protein (GFP) gene was transferred into the FLSPCs. Animal models of drug-induced acute liver failure were made with carbon tetrachloride by peritoneal -7- 第四军医大学硕士学位论文 injection. The partial hepatectomies with 85% sizes were performed on the mice to produce models of acute liver failure of injury.After 12 hours the FLSPCs transfected successfully by GFP were transplanted into treated groups through caudal veins. Observe the liver-repair condition at different points . Differences are significant in hepatic functions (ALT, AST, TBIL and ALB) between treated groups and control groups 12 hours after transplantation. Compared to control group the in