黄连吴茱萸不同比例配伍对寒热错杂型功能性消化不良大鼠胆碱能神经系统的影响机制分析-方剂学专业毕业论文.docxVIP

成都中医药大 成都中医药大 PAGE 10学硕士论文 实验方法对大鼠功能性消化不良模型造模成功；②各造模组大鼠胃排空能力明显 降低，与空白组比较差异显著（P＜0.05）；③经连萸水煎液治疗后，黄连吴茱 萸（1:3）能提高乙酰胆碱、乙酰胆碱酯酶、乙酰胆碱转移酶在血液中的含量， 与模型组比较差异有统计学意义（P＜0.05）。 【结论】：①本实验造模方法能够导致大鼠出现功能性消化不良（寒热错杂型） 症状表现；②黄连、吴茱萸配伍对本实验模型大鼠通过增强胆碱能系统的功效， 在治疗功能性消化不良方面具有明显优势。黄连吴茱萸配伍体现了辛开苦降法配 伍理论，其增强胆碱能系统功效的实验结果和作用机理间接说明这一理论具有 物质基础。 关键词：黄连；吴茱萸；功能性消化不良；乙酰胆碱；乙酰胆碱酯酶；乙酰胆 碱转移酶 2 ABSTRACT Objective :Research carrier of cold and heat mixed type functional dyspepsia rats, a model drug and research object berberine Evodia compatibility, blood acetyl choline, acetyl choline ester and acetyl choline transfer enzyme content as the focus of inspection, by means of rigorous scientific animal experiments results deepenthe berberine Evodia compatibility understanding, reveal the treatment of functional dyspepsia mechanism of action and the scientific connotation to expand its clinical application. Methods :①Experiment 1: 100 Kunming mice (male and female), were randomly divided into 10 groups. Blank control group and the pathological model group was given the same volume of saline, and the remaining eight were fed with berberine, Evodia different proportions suspension, cisapride tablets suspension continuous administration 5d. (The night before the fifth day administration 1h help but water fasting 12h), in addition to blank group modeling, and the remaining nine groups are established intraperitoneal injection of atropine delayed gastric emptying in mice model. Each group to the nutritional semi-solid paste 20min after taking the cervical vertebrae were killed, anatomy, remove the full stomach and intestines. Weighing and record the net weight of the full weight of the stomach and stomach, and then calculate the rate of gastric emptying; taken from the pyloric part of the intestine to the rectum, measuring the length of the intestinal length and nutrition solid paste in the intestine to promote, record data and calculate the rate of intestinal propulsion ②.Experiment 2: 8-week-old Wistar rats were 80 m