磷酸盐快速检测试剂盒的研发及应用-轻工技术与工程（发酵）专业毕业论文.docxVIP

ABSTRACTPhosphate ABSTRACT Phosphate is an essential element of microbial cells，involved in the synthesis of phospholipids，nucleic acids and a variety of coenzymes，it also plays 8．11 important role in HMP and EMP which are the core glucose metabolic pathways．Phosphate metabolism is of great importance in controlling the growth metabolism and metabolic regulation of microbial cells and thus monitoring its contents provides a basis for establishing novel control methods in fermentation processes．In this thesis，a rapid phosphate detection method was developed and its applications to analyzing the relations between phosphate consumption，cell growth and products synthesis were conducted．The main results were aS follows：Through investigating the operation parameters，the quantitative method for rapid phosphate detection was optimized，leading to the development of a novel phosphate rapid detection kit．Its lower limit of quantitation and the optimum pH range were 9．75x 10。8 mol L“and 4．5～7．6．respectively．The detection error is 1-3．2％．The pretreatment method was also determined as follows：(1)the sample is mixed with equal volume of 2 mol L_NaOH and allows standing for 1 0 min；(2)1／2 volume of 1 mol L_HCI is then added to dissolve the precipitates at 60。C；(3)2 volume of anhydrous ethanol is finally added．After 1 0 minutes’standing．the supernatants are collected by spinning at 1 2000 r min一1 for 5 min． The kit Was used to quantitatively analyze the phosphate contents in several commonly used materials for inedia including yeaSt extract，peptone and com steep powder and in Escherichia coli，Bacillus licheniformis and Aspergillus niger．The results were in good agreement with those obtained by the national standard method． Recombinant E coli 42—0撑fermentation was used as a caSe study for evaluating the utility of the kit for monitoring the dynanfics of phospate contents in the fermentation process as well aS to analyze the corresponding relationships between cell growth，enzyme prod