三种植物的组织培养及生根过程中酶活性的变化-植物学专业毕业论文.docxVIP

摘 摘 要 本论文以紫叶挪威槭、一品红、萱草为材料，进行组织培养，建立再生体 系。研究了植物生长调节剂DPC和复合VB对一品红试管苗生长、芽增殖及生 根的影响。研究了三种植物生根过程中IAAO、POD活性的变化及POD同功酶 酶谱的变化。研究结果如下： 1．紫叶挪威槭最佳芽增殖培养基为MS+BAl．5mg／I．,(单位下同) +ZT0．I+NAA0．I+GA0．5+CH300，芽增殖率为4．35：最佳生根培养基为MS+ NAA0．I+IBA0．5+PG40，生根率为72．4％。一品红最佳芽增殖培养基为MS+ BAl。弧lBA0。1，增值率为7．87；最佳生根培养基为1／2MS+IBAl．0，生根率为 82．6％。萱革最佳分化培养基为MS+BAl。0+KTl．0+IBA0．5，分化率为93。3％； 最佳生根培养基为1／2MS+IBA0．5，生根率为100％。 2．培养基中DPC浓度为5mg／L时，利于芽的增殖；浓度为10mg／L时，试 管苗生长健壮；浓度为30mg／L时，矮化作用明显。复合VB浓度为0．5-1．0mg／L 时，苗高显著增加；浓度为0．2-0．5mg／L时，促进根的分化，提高生根数量。 3．萱草和一品红IAAO、POD活性交化趋势有相似之处，IAAO活性先升高 褥降低，之后回舞；POD活性剐先降低后升高，之后略有下降。紫叶挪威槭生 根诱导初期IAAO、POD活性大体都呈缓慢上升趋势，后期活性降低。当IAAO 和POD在各自的动态曲线中处于相对低水平时有利于生根。紫叶挪威槭IAAO、 POD起始活性较高，推测试管苗生根培养起始时期酶活性的差异可能也是造成 不同植物生根难易的一个影响因素。生根过程中POD同功酶酶谱发生变化。 关键词：组织培养；生根；嬲l哚乙酸氧化酶；过氧化物酶 AbstractIn Abstract In the study,Acer platanoides‘Crimson磁蜡’，Euphorbia phlcherrima and Hemerocallis hybrida were used as explants for tissue culture and the efficient plant regeneration system were established．At the same time，the effects of different plant growth regulators on growing，shoot propagation and rooting of Euphorbia phlcherrima were studied．Finally,we studied the changes of enzyme activity and peroxidase isozyme spectrum during rooting． The results showed that the most suitable mediun for shoot propagation ofAcer platanoides‘CrimsonⅪng’was MS+BAl．5+ZT0．I+NAA0．I+GA0．5+CH300 and the shoot propagation rate was 4．35；The best mediun for rooting Was MS+NAA0．1+ mA 0．5+PG40 and the rooting rate was 72．4％．The best medium for shoot propagation of Euphorbia phlcherrima Was MS+BAl．0+IBA0．1 and the shoot propagation rate Was 7．87；The promoting root medium was 1／2MS+IBAl．0 and the rooting rate Was 82．6％；The ideal medium for differentiation of adventitious buds of Hemerocallis hybrida was MS+BAl．0+KTl．0+IBA0．5 and the differentiation rate was 93．3％；The medium of 1／2MS+IBA0．5 Was proper for rooting of tube seedlings and the rooting rate was 100％． The results showed that DPC of lower concentration had effect on enhancing multip