流脑W135群,Y群结合疫苗的初步研制-生物化学与分子生物学专业毕业论文.docxVIP

摘要摘要 摘要 摘要 流行性脑脊髓炎(meningococcal meningitis)简称为流脑，是由脑膜炎双球菌引 起的急性化脓性脑膜炎。目前的流脑疫苗分为多糖疫苗和结合疫苗两大类，多糖疫苗 的主要成分为从脑膜炎球菌中提取的荚膜多糖。多糖疫苗为非T细胞依赖的抗原。对 于二岁以下婴幼儿免疫原性很差而且在所有年龄群体中都不产生免疫记忆。结合疫苗 为荚膜多糖与蛋白载体偶联形成的，结合疫苗为T细胞依赖的抗原，在人体内能产生 免疫记忆。 国内尚未报道有以CRMl97为蛋白载体进行流脑W135群，Y群结合疫苗的研究。 本实验成功的进行了W135群，Y群结合疫苗的初步制备，建立了稳定的发酵工艺以 及环保的荚膜多糖纯化工艺。通过DOC以及Tritonx．114两种方法有效的去除了内毒 素。初步制备了荚膜多糖跟CRMl97的结合物，通过动物试验证明是成功的。 本研究为流脑W135群，Y群结合疫苗的研制打下了基础，同时也为A，C，W135， Y群四价流脑结合疫苗的研制奠定了基础。 关键词：流脑结合疫苗，CRMl97，荚膜多糖纯化 AbstactNeissefta Abstact Neissefta meningitidis causes meningococcal meningitis．There are two kinds of vacclnes and vaccines against meningoc幻ccal meningitis，including polysaccharide are coniugate vaccines．The major components of polysaccharide vaccines p01ySaccharide extracted from Neisseria meningitidis．Polysaccharide vacclnes盯e are not T-cell．dependent antigen．It was found that most polysaccharide vacclnes poorly inmlunogenic in young infants and children less than 2 years old and fail to induce immun010西cal memory in people of any age．Conjugate vaccines reter to vaccines of which capsular polysaccharides conjugated to protein carriers·Conjugate vaccines are T-cell．dependent antigen，which can produce immune meomory· Bv nOW,it has been reported that there is no research about meningococcal groups W1 35 and Y polysaccharide--protein conjugate vaccine with C刚197 aS camer protein in China．Conjugate vaccine groups W1 3 5 and Y was successfullY pr印ared in this study．Stable fermentation process and environmentallY benl印ed was p嘶fication process of capsular polysaccharides were established·Endotoxin e彘ctivelv removed by the treatment of DOC and Tfitonx。1 1 4·Animal test data has shown that preliminary preparation of conjugate vaccine lS SUCCe：88tul· The research lays a foundation for the meningococcal groups W1 3 5 and Y p01ysacch撕iIe呻mtein conjugate vaccine development， which will lead to a quadrivalent meningococcal glycoconjugate vaccine against memngococcal serogroups A，C，W1 35，and Y． Keywords：meningo