声刺激对两种海洋甲壳动物运动行为和HSP70表达的影响研究-海洋物理专业毕业论文.docxVIP

摘要时频域特性、声传播衰减等参数； 摘要 时频域特性、声传播衰减等参数； 3、在实验室环境下，设计了不同强度、不同类型水下噪声源及其对海洋甲 壳动物影响的模拟实验方法。鉴于运动行为和HSP70在南美白对虾和拟穴青蟹 日常行为活动及生理活动中扮演的重要角色，分析了南美白对虾和拟穴青蟹的运 动行为以及拟穴青蟹HSP70的表达，并以运动参数和HSP70表达的变化作为南 美白对虾和拟穴青蟹是否受噪声影响的判断依据； 4、通过对实验组和对照组南美白对虾运动轨迹的记录和运动参数的统计分 析，探究了水下打桩噪声对南美白对虾的运动行为影响； 5、通过对实验组和对照组拟穴青蟹运动参数的统计分析及HSP70表达的测 量，探究了在不同噪声源(频带在100．1000Hz的扫频噪声以及模拟水下打桩噪声) 强度的声刺激下，拟穴青蟹的运动行为及HSP70表达的变化，分析噪声对其影 响。 关键词：南美白对虾：拟穴青蟹：声刺激；运动行为；HSP70表达 II 万方数据 AbstractAbstract Abstract Abstract Underwater noise has the potential to bring stress to marine animals，distract and mask important sounds，and thus affect marine animal behavior and physiological parameters．Along with the development of human society,the human has given a new focus on exploring ocean resources．In recent decades，with the rapid development of the marine industry,the effect of man-made noises marine life has attracted great attention．Many studies have evaluated the effects of anthropogenic acoustic disturbance on marine organisms，including sounds associated with shipping， pile driving,sonar,offshore wind farms and many other anthropogenic sources．The results showed that，these man-made noises have caused different degrees of influence on fish and marine mammals．However,few studies have evaluated the effects of acoustic stimuli on aquatic erustaceans，which are indispensable to the food chain of marine life． There is a great variety of marine crustaceans，many ofthem are important edible species，and have great economic value．Among them，Litopenaeus Vannamei and Scylla paramamosain have unshakable status，because of their economic value， medicinal value and the value of new natural products．Meanwhile，research has found that many kinds of marine crustaceans could make sounds by moving，vibrating or rubbing their body,and also can receive and use acoustic signal．Considering aquatic crustaceans had been studied hearing are sensitive to acoustic stimulus below 1 000Hz in frequency,the noises energy mainly concentrate below l 000Hz maybe constitute