农学院2009年共发表SCI论文130篇详细情况如下.DOCVIP

农学院2009年共发表SCI论文130篇，详细情况如下： 1. 【篇名】Purification of a bioactive recombinant human Reg IV expressed in Escherichia coli 【作者】Hu, G. Y.,Shen, J. Q.,Cheng, L.,Xiang, D.,Zhang, Z. H.,He, M.,Lu, H. L.,Zhu, S. Y.,Wu, M. Y.,Yu, Y.,Wang, X. P.,Han, W. 【期刊】Protein Expr. Purif.,69(2) 【摘要】Regenerating gene (Reg) IV is a newly discovered member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily. Reg IV is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. However, the physiological and pathological functions of Reg IV are largely unknown, partly due to the limited access of the bioactive protein. We report here the first expression and purification of Reg IV proteins using a prokaryotic system. Human Reg IV was expressed in Escherichia coli as an insoluble protein which was identified in the fraction of inclusion body after ultrasonication of the bacteria. After the protein aggregate was solubilized by guanidine-HCl, it was refolded by sucrose and arginine-assisted procedures and purified using cation-exchange chromatography. The protein identity and purity of the final preparation were confirmed by analysis of the protein mass and immune specificity in SDS, Western blotting, and HPLC assay. The biological activity of the protein was determined by the HCT116 and HT29 cell proliferation assays. The highly purified bioactive human Reg IV should aid in further characterization of its physiological and pathological functions. (C) 2009 Elsevier Inc. AM rights reserved. 2. 【篇名】YIELD, FRUIT QUALITY AND NITROGEN UPTAKE OF ORGANICALLY AND CONVENTIONALLY GROWN MUSKMELON WITH DIFFERENT INPUTS OF NITROGEN, PHOSPHORUS, AND POTASSIUM 【作者】Song, S. W.,Lehne, P.,Le, J. G.,Ge, T. D.,Huang, D. F. 【期刊】J. Plant Nutr.,33(1) 【摘要】The effects of varied amounts of fertilization on yield, fruit quality, and nitrogen (N) uptake of muskme