离子液体对简单节杆菌特性的影响-发酵工程专业论文.docxVIP

ABSTRACTIn ABSTRACT In this study，tlle biotransfomation of cortisone acetate(CA)by么，扬，D6口c纪，sf彬pZ蹦 口田’)was selected aS a model system for eValuating the innuences and material basis of Ils on me characteriStics of彳SP．The traIlSport characteristics of difjFerent reaction medium， including【PrMIlll】PF6，HP-p-CD，Tween80，hexadecalle，was also aJlalyzed． ARer adding ILs in fementation culture stage，the gr0、Ⅳth of bacteria aIld metab01ic ability were declined of Varying degrees．Among them，ILs，which side cham au(yl carbon atom n啪ber waS 2，had smaller innuence bacteria．When the side chain alkyl carbon atom number became 3，the influence on bacteria challge酎eatly，compared witll controls， biomass reduced to 54％，sugar metabolism Vigor drops to 55％．When the side chains of carbon atoms n删曲er3，bacteda掣o、矾h was almoSt completely inhibitted，metabolism ability only remain at around 20％．For the same ILs，the toxicit)，of bacteria is proponional to the inceeased ILs concentration． With or witllout ILs pretre at]【11ent，compared the process of the l—en-dehydrogenation of CA in彳iSP．The ceU properties haVe no obVious change when side chain alkyl carbon atoms n啪ber is 2．after pretreatmem by【PrMIm】PF6，side chain alkyl carbon atom number is 3，t11e CA conversion rate increaSe nearly 1 0％，besides it has most obVious solubilization ef．fect on t11e substrate，nearly 2-南rd higher than tllat of control，expressed lower dehydrogenaSe actiVi够，dropped out 20％，and higher ceU pe肌eability，under the dual role of in the solubilization ef-fect aJld me change of cell characteristics，conVersion rate increase significantly． The micro孕印hs perfomed by TEM illustrated that aRer being仃eated by ILs．7rhe cells pre-incubated witllout ILs had a t)，pical么即ultraStmcture．Cells remained intact，and the cell enVelope was complete aIld clear．When the cells were exposed to 2．5％(V)ILs，the ceU envelope bec锄e bluHed and lost its inte西够．Cell pemeability is closely related to the integrity of ceU membrane．Thus，these alterations may ex