雷公藤有效成分分离研究-化学工程专业论文.docx

浙江大学博：t学位论文Abstract 浙江大学博：t学位论文 Abstract Triptery#um wilfordii Hook．f．frWhr)is a traditional Chinese herb grown in the south of China and used for various immune and inflammatory diseases for many years．The extracts ofthe roots ofTWHF are used widely for the treatment ofa number ofautoimmune disorders such as rheumatoid arthritis，organ transplantation，systemic lupus erythematosus,and skin diseases．The main active constituents of the herb have been reported to he diterpenes， triterpcnes and alkaloid’s compounds．In this dissertation,a series of rapid,sensitive and accurate LC／MS analytical methods were established for the determination of triptolide， tdpdiolide，wilfoarine，wilfordine，wilforgine and wilforine in extract of TWHF．The separation and isolation of triptolide，tdpdiolide，wilfortrine，wilfordine，wilforgine and wilforine from TWHF is emphasized．m this dissertation．The main contents ofthis work arc as following： (1)A novel method using liquid chromatography(LC)coupled with all atmospheric pressure chemical ionization mass spectrometry(APCI-MS)iIl the negative mode has been developed and validated for the rapidly simultaneous determination oftriptolide and tripdiol池． (2)The first analytical procedure determination of wilfortrine—l，wilfordine-2， wilforgine-3 and wilforine-4 is described．Identity ofthe analytes was confirmed．m a LC．MS experiment，with all compounds being clearly assignable．m an atmospheric pressure chemical ionization(APCO positive mode． (3)The sample preparation of crude triptolide from leaves of TWHF was optimized．A single—step preparative high-speed counter-current chromatography(HSCCC)method was developedfortheseparationandpurificationoftriptolidefromthe extractofleavesofTWHF． The ratio of compositions of the solvent system composed of petroleum ether-ethyl acetate— ethan01-water were optimized．The effects ofrotation speed ofthe multilayer coil column and flow rate ofmobile phase on the retention ofthe stationary phase were investigated． (4)In col