利用issr和rapd对白桦纤维长度性状的标记研究-林木遗传育种专业论文.docxVIP

AbstractIn血is Abstract In血is paper,we measured the fiber traits of 583 birch materials and chose l 00 individuls by ISSR markers and RAPD markers．After surveying with ISSR and RAPD primers,1 0 fragments from ISSR and RAPD primers were identified having significant correlation with the length of fiber traits based on correlation coefficient analysis and Successfully transformed into SCAR markers．and the results are as follows： 1、From the surveying result of 583 materials，the fiber traits were showed the normal distribution．And we chose 100 individuls to be analysed by genomic study． 2、We improved original method of CTAB．this improved method was not only suitable for birch genomic DNA extraction but alSO for other paints．This extraction method was multi．times STE—CTAB．It WaS easier and faster,operated more easily, extraction condition was gentler,the biochemical and instruments were very normal．The most important Was high purity DNA，and could be directly used to PCR ampli母ing experiment． 3、The best suitable reaction system of ISSR includes 2．5 nunol·L”M92+．1 O rnmol’L-‘dNTp．10 mmol‘L。primer,1 xTaq DNA polymerase buffer,and 45 ng template DNA in a total volume of 20 uL．ISSR．PCR reaction system of birch was performed． Initial denaturation was for 5 min at 94℃。follow by 30 cycles ofdenaturation for 30 S at 94℃。annealing for 30 S at 51℃．extension for 30 S at 72℃and a final 7 min extension at 72℃fYang Chuanping et a1．2005)．The PCR products were isolated by electrophoresiS and photographed with UVP Gel Documentation Systems(GDS7600)．Molecular weight was estimated using a 1 00 bp DNA ladder(MBI)．RAPD reaction system is the SalTle to ISSR system．but RAPD．PCR reaction systcm of birch Was performed+Initial denaturation Was for 3 min at 94*C，follow by 40 cycles of denaturation for 60s at 94℃，annealing for 60 S越37℃．extension for 2 min at 72℃and a final 7 min extension at 72℃． 4、Further analysis in 1 00 birch varieties revealed that correlation coefficient was much closed to the s