临床分离鲍曼不动杆菌对氟喹诺酮类抗菌药物耐药性检测和耐药基因研究-临床检验诊断学专业论文.docxVIP

安徽医科大学硕士学位论文 安徽医科大学硕士学位论文 — —6— ％、24.2％、12.5％respectively．The resistance rate of Acinetobacter baumanii isolates to Ciprofloxacin，Levofloxacin and Gatifloxacin were 78.3 ％、58.3 ％和 40.0％ respectively．multi-drug resistance Acinetobacter baumanii(MDR-AB)have a higher resistance rate than others． Polymerase chain reaction(PCR) and DNA sequencing indicated that all strains of Acinetobacter baumanii have PCR products of gyrA and parC genes ,but no products of qnrA,B,S and qepA genes on plasmid；the postive rate of PCR products of aac(6’)-Ib is 29.2%（35/120），but there were no aac(6’)-Ib-Cr genes on plasmid by DNA sequencing； there were the point mutations in gyrA genes at codon 83 of quinolones resistant strains but no such mutation in sensitive strains．We found the new point mutations in gyrA and parC genes of resistant strains to that with the Genbank number of DQ270238 and EU977576，respectively． Conclusion ： The current status of resistance to quinolones among Acinetobater baumannii and MDR-ABA strains in Hefei region of China is serious，and the resistance is related to the point mutations within gyrA and parC genes，but isn’t related to genes qnrA、B、S、aac(6’)-Ib-Cr and qepA on plasmid．Surveillance of bacterial resistance is of great important to rational selection of antibiotics and preventing the abuse of antibiotics and the emergence of new drug-resistant strains． Key words：Aeinetobacter，baumanii；multi-drug resistance(MDR)；antimierobial agent；gene；quinolones；resistant mechanisms — — PAGE 1— 中英文缩略词表 缩写词 英文全称 中文名称 resistant 耐药 I intermediate 中介 susceptible 敏感 MIC Minimum inhibitory concentration 最低抑菌浓度 CLSI/NCCLS Clinical and laboratory standards institute  美国临床及实验室标准协会 MDR multi-drug resistance 多重耐药 AB Acinetobate baumannii 鲍曼不动杆菌 QRAB Quinolone-resistant Acinetobacter baumannii  氟喹诺酮耐药鲍曼不动杆菌 RND Resistance-nodulation division family  耐药结节细胞分化族 QRDR Quinolone resistance determining region  喹诺酮耐药决定区 AMK Amikacin 阿米卡星 CIP Ciprofloxacin 环丙沙星 LVX Levofloxacin 左