裂褶菌胞外多糖的研究-发酵工程专业论文.docxVIP

天津科技大学硬士学链论文攮 天津科技大学硬士学链论文 攮 要 本文对裂裙菌施井多糖韵发酵条件，提取工艺及结构迸行了研究，确 定了裂褶蘩产糖静最佳培养蒸、涪养条件、提取工茳条件及多糖韵结构。 研究懿主要内容释结论据下： 1。在裂褶甍藏崧多糖懿发簿研究中通过擎因子试验帮正交试验确定了裂 耱藏产糖静最优培养蒸配方为：葡萄糖459／L，酵母膏39／L，KFI：PO。 0。59／L，MgSO。·7FI。0 0。59／L；最遥发酵条传为：接释龄66h，接释量 10％，壤葵瀑度27℃，装液量20％，秘始pH自然。褥到摇糕条件下菡体 最大{!譬搴20。759／L，腿终多糖最大褥率2．709／L。经7毛发释罐熬分批发 酵褥至B蓝丝于羹产搴为8。819／L，腿终多糖产率必0。849／L。多糖与菌体 比为1：10。枣}水壤驿发黪试验结果必薰丝于重褥率t9。989／L，裂褶萤 胞夕}多糖褥率l。899／L，分别必分批发酵麴2。27馈秘2．25倍，露多糖 萤体比朱变。 2．对裂褶菌胞外多糖的提取工艺进霉亍了初步磷究。确定了最佳的脱色方 法为活性碳吸附法，最适加量为0．5％。最佳脱蛋白方法为等电点法与 Sevag法2次棚结合的方法。工艺路线为：发酵波一除去萤体一堰烛碳脱 色一浓缩一脱蛋白一乙醇沉淀一复溶一沉淀一纯裂褶菌胞外多糖 3．进行了裂褶菌胞外多糖的结构分析。由凝胶柱腰析，聚丙烯酰胺凝胶 电泳，高效液稠色谱分析可知裂褶菌胞外多糖为均一组分，分子量4× 104u。通过完全水解，纸层析，气相色谱分析单糖组分，红外光谱，酶 解爱应，高磺酸氧化和圆体核磁共振分析结构，证明了裂褶菌胞矫多糖 是菇葡萄糖为攀一组分的tB一(i一3)一D葡聚糖，其中每三个葡萄糖分子上 涟青一个t3一(卜6)翡分支。 失键谒：裂摆蘩，裂褶藩黢终多糖，发酵，提取，结构鉴定 ABS_H澉TABSTRAC ABS_H澉T ABSTRAC T The fcrmentation conditions，the extraction methods and the structure of schizophyllan were studied in this thesis．The main contents and results were as follows： Fermentation schizophyllan by Schizop砂llum commune was optimized．The results of single factor and orthogonal experiment showed that the optimized medium composition contained：glucose 459／L，yeast extract 39／L，KH2POa O．59／L，MgS04．7H20 0．59／L．The optimized conditions were inoculum age 66h，inoculum size 1 O％，temperature 27 ℃．volume of medium 20％and pH 6．Maxium yield of schizophyllan and mycelium of flask were 2．709／L and 20．759／L respectively．But the yields of schizophyllan and mycelium were O．849／L and 8．819／L respectively in batch culture．The result of water-fed experiment showed that the yields of mycelium and schizophyllan were greatly improved and them were 19．989／L and 1．899／L respectively． The extraction method of exo—polysoccharide from schizophyllum commune by submerged cultivation was studied．The extraction technology was improved and the best methods of removing color and Proteins were achieved． The structure of schizophyllan from schizophyllum commune by submerged cultivation was also researched，The purified sch