利多卡因对急性肺损伤中肺组织细胞凋亡的影响-麻醉学专业论文.docxVIP

徐州医学院硕士学位论丈The 徐州医学院硕士学位论丈 The effect of lidocaine on apoptosis of lung tissue in acute lung injury Abstract Objective To examine the anti—apoptosis effect of lidocaine on lung tissue in acute lung injury,and to explore the possible mechanism． Methods Male Sprague—Dawley(SD)rats were divided into four groups randomly They were saline control treated group(I尚)，lidocaine control treated group (n《)，lipopolysaccharide(LPS)treated group(n=6 each)，and lidocaine+LPS treated group(n=6 each)，respectively．All groups were subjected into 6 h，12 h，and 24 h point subgroups after LPS adminstration，except of saline control treated group and lidocaine treated control group．The lung wet-to—dr)，weight ratio and the histopathologic changes of lung tissues were detected．And apoptotic cells were stained by terminal deoxynucleotidyi transferase—mediate dUTP nick·end labeling(TUNEL)technique．To explore the related molecular mechanism，the protein expression level of Fas in lung tissue was examined by Westem blot．Further,to confirm the role of lidocaine in this model，the mortality of rats in LPS group VS lidocaine+LPS group was compared． Results lung wet··to·-dry weight ratio Was significantly up··regulated after LPS administration．H—E stain of lung tissue showed edema(both interstitial and alveolar) and neutrophils infiltration in alveolar tissue．Alveolar spaces were narrowed and erythrocytes exuded．When the tissue sections were labeled with an in situ TUNEL assay to detect apoptotic cells in lungs，no TUNEL—positive cells were observed in saline control treated group and lidocaine contrlo treated group．Induction of sepsis by LPS resulted in a marked appearance of TUNEL—positive cells．The expression of Fas was substantially increased．Pretreatment with lidocaine，the apoptosis of alveolar wall ～ cells was reduced．Western blot analysis showed lidocaine inhibited the expression of { Fas in lung tissue． 0 Conclusion lidocaine attenuates LPS—induced acute lung injury and reduces alveolar wall cell