酪氨酸酶抑制剂的开发和研究-化学生物学专业论文.docxVIP

第四章从现成的天然产物和常见的金属离子和酸根离子中，筛选出效果好的酪氨酸酶抑制剂。 第四章从现成的天然产物和常见的金属离子和酸根离子中，筛选出效果好的 酪氨酸酶抑制剂。 第五章利用筛选得到的效果较好的天然产物和金属离子，做初步的相互 之间的协同抑制研究。 关键词：酪氨酸酶，酪氨酸酶抑制剂，香豆素，天然产物，金属离子，酸根离子， 协同抑制 lI Title：Development Title：Development and study on Tyraseosinase inhibitors Maj or： Chemical Biology Name：Jialiang Shao Supervisior：Prof．Lin Ma Abstract Tyraseosinase(monophenol monooxygenase EC．1．14．18．1)，a copper-containing enzyme，catalyzes two distinct reactions of melanin synthesis，the hydroxylation of a monophenol and conversion of an o—diphenol to the corresponding O-quinone． Tyraseosinase widely exists in plants and animals，and is involved in the formation of melanin pigments．In the food industry,Tyraseosinase is a very important enzyme in controlling the quality and economics of fruits and vegetables．Tyraseosinase catalyzes the oxidation of phenolic compounds to the corresponding quinones and is responsible for the enzymatic browning of fruits and vegetables．Tyraseosinase inhibitors have become increasingly important in medicinal and Cosmetic products， primarily in relation to hyperpigrnentation．Therefore，development of highperformance Tyraseosinase inhibitors has strongly been required in agricultural and food fields． In this thesis，in order to develop and study丽m Tyraseosinase inhibitors，several series of coumarin derivatives were designed and synthesized based on the structural features of the enzyme．The biological activity of the coumarin derivatives were investigated，too．Furthermore，more than 90 natural products，some familiar metal ions and acid radical ions were screened，several good inhibitors were found． 11I This This thesis was divided into five major parts． In Chapter one，the functions and structure of Tyraseosinase were introduced． Moreover the latest researches on Tyraseosinase inhibitors were reviewed． In Chapter two，a series of aryliminobenzofurocoumarin derivatives were designed and synthesized，and were studied on their Tyraseosinase inhibition activities． In Chapter