利用RAPD分子标记对杂交水稻DNA多态性的研究-作物栽培学与耕作学专业论文.docxVIP

POLYMORPHISM POLYMORPHISM oF THE PARENTAL LINEES IN HYBRID RICE(Oryza．sativaL．)BY RAPD MARKERS Abstract Twelve rice male sterile lines，9 maintainerblines and 15 restorer lines were analyzed by RAPD with 10primers．altogether,106 fragments were generated，of which 81 detected polymoprphism among rice lines tested．Cluster analysis showed that all lines could be uniqeuely distinguished by RAPD marker． In order to obtain a stable RAPD reaction system，we optimized the reaction conditions based on experimental results．The results showed that the reaction system，which included 10～60ng DNA，2．0mmol／L Mg”，1．5-2．0mmol／L dNTPs， 1．2—2．0 u mol／L random primers and O．8—1．2U Taq polymerase·WaS good for our experiments．Ten primers，which produced repeatable，clear and rich amplified polymorphism in the RAPD analysis，were obtained by screening 40commercial primers．They are$227，$228，$229，$290,$358，$400，$490，$1405，S1422and S1492． Based on the pair-wise comparsons of amplification products the genetic distance WaS calculated using NeiLi’S genetic similarity coefficient and a dendrogram WaS constructed using an un-weighted pair group method with arithmetical averages(UPGMA)，the result that the genetic similarity coefficient was very identical and the genetic difference of 36 rice lines was very small．their genetic polymoprphism was distinguishable，even the genetic backdrop among the male sterile lines and maintaier lines was very similarity,their genetic polymoprphism was also distinguishable． Key words：hybrid rice；RAPD marker；polymoprphism；genetic relationship 广西天学疆土学位论文·,瘛hWRAPD静子褥记对赦交承稻0黼爹拣褴魏研究i前言 广西天学疆土学位论文·,瘛hWRAPD静子褥记对赦交承稻0黼爹拣褴魏研究 i前言 1．1 遗传标记的发展概况 麓了矮手获鼗骤表瑷鳌懿表遮寒谈谖基嚣鍪，楚蘧黪逡传努耩豹受遗健控裁 的表现型差异，染色体的行为和结构的差异以及DNA片段的差异称为遗传标 记(genetic markers)眠遗传标记在遗传图谱的构建、基因定位、标记辅助育种、 系统避纯、速簧多撵经露蘩矮撩定等酝究孛怒藿重要终溪。遗簧蠢记(genetic markers)应用促进了遗传育种学的发展，它先厢经历了形态标记(morphological markers)，细胞标记(cytological markers)，生化标记(biochemical markers)和分 子拣铤fm鹾e秘斑markers)酉个狳毅。 1．1．1形态标记 形态标记是从澎态学或表型性状上来检测遗传变异，魑指生物特定的肉眼可 蠢戆终罄穗薤蒋戆，