辣椒碱类化合物及辣椒碱单体的提取与纯化-生物化工专业论文.docxVIP

2007届浙江大学颤1．毕业论文辣擞碱类化合物及辣椒碱荦体的提取+J纯化 2007届浙江大学颤1．毕业论文 辣擞碱类化合物及辣椒碱荦体的提取+J纯化 和流动相流速等对分离的影响，确定了适宜的f=艺操作条件：流动相为甲醇／水 (75／25，v／v)，进样浓度为180mg／ml，进样体积为2．08ml，流速为4．Oml／min。 在该工艺条件下，得到的辣椒碱单体纯度为95．23％，酬收率为93．27％，产率为 4．389／(L．h)，流动相消耗为O．087L／g。本方法操作简便，分离效率高，产品质量 好，为辣椒碱单体的制备研究提供了一种较好的方法。 全文建立了辣椒碱类化合物及辣椒碱单体的HPLC分析方法。 关键词：辣椒碱类化合物辣椒碱单体提取大孔吸附树脂结晶反相型高 效液相制备色谱 n 2007届浙江大学颂I．毕业论文辣椒碱炎化合物肢辣搬碱中体的提取‘j纯化 2007届浙江大学颂I．毕业论文 辣椒碱炎化合物肢辣搬碱中体的提取‘j纯化 ABSTRACT A process for extracting and purifying capsaicinoids and capsaicin from capsicum oleoresin was studied in this paper．The process includes extracting coarse capsaicinoids by solvent，purlfying capsaicinoids by macroporous resin and erystalliz- ation，and preparating capsaicin by reversed-phase preparative liquid chromatog- raphy． In the experiments of ex仃acfion of coarse capsaicinoids by solvent,the optimal operation conditions were obtained by studying the effects of saponification and extraction on the purity and recovery．Firstly,capsicum oleoresin was saponified by a sodium hydroxide solution of 5％content．and the recovery was 98．74％while purity of capsaicinoids was improved from 34．89％to 65．20％．Secondly,the pH of saponified solution was conditioned to 9．57 and extracted with ether two times to increase the purity to 90．49％with a recovery of 98．75％．Thirdly,a 2％sodium hydroxide solution extracted the above ether mffinate three times and the purity was improved to 94．52％with a recovery of 91．14％．Through the three processes。the capsaicinoids of 94．52％could prepared from capsicum oleoresin with the total recovery of88．87％． In the experiments ofpurifying capsaicinoids，the methods ofmacroporous resin and crystallization were studied．In the method of macroporous resin,the effects of the contention，flow rate，pH value of capsaicinoids on the adsorption and the concention，flow rate on the desorption were studied．The optimal operations conditions were obtained：loading flow rate：1．91BV／h；loading sample pH：12．3 O： eluant：firstly 20％acidic ethanol solution，