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high concentration could the ammonia inhibit the nitrogen excretion of juvenile mullet that fed with the same level of food, the nitrogen excretion between control and low-concentration group was not significant; due to the increase of exogenous nitrogen metabolism, the growing ration size could increase the nitrogen excretion significantly. Growth was significantly influenced by ration size and ammonia exposure, as the control and low-concentration group performed better than high-concentration group in
terms of special growth rate (SGR,include SGRw and SGRe) under the same ration size;
meanwhile, the special growth rate (SGR) of juvenile mullet displayed even better with the increase of ration size under each ammonia concentration. The difference in growth performance is mainly attributed to variations in food intake and food conversion efficiency (FCE).
(3) The effects of ration size and ammonia exposure on two enzymes in juvenile mullet In present study, the effect of ammonia exposure on the pepsin of juvenile mullet
was different from each ration size, only the pepsin activities of juvenile mullet from high level feeding group was depressed by the high concentration of ammonia, showing a possible relation with food intake; the pepsin activities of juvenile mullet under control and low-concentration were significantly impacted by ration size while under high concentration the influence was not significant. The food intake proves to be the main factor that influences the pepsin activity, and the high concentration ammonia may influence the pepsin activity indirectly by inhibiting the food intake of juvenile mullet.
The general trend of GS activities in gill and muscle of juvenile mullet was the same. The GS activities of median and high-level feeding group was induced by ammonia; the GS activities of control and low-concentration ammonia group displayed a significant increase with the rise in ration size, while in high -concentration group the difference was not
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