缩宫素粗品的分离纯化新工艺研究.pdf

liquid crude as well as separated oxytocin sample. (2) Discussed the retention mechanism of Oxytocin on polyamide column, by different concentrations of acetonitrile isocraticaly elute on polyamide column. The results shows to be mix-mode retention behavior of the hydrogen bond adsorption and hydrophobic interaction. (3) Established and optimized pure water isocratic elution method for the separation of the oxytocin solide crude on polyamide. This method could achieve good desalination and the crude separation result. Under the optimized separation conditions, the separation result of the solid crude could up to a purity of 65.8%, with the recovery of 72.1%. Compared with the traditional low-pressure reversed phase C18 desalting method, the polyamide method has a higher sample capacity, a lower separation and purification cost, and is good to the environment. (4) The adsorption mechanism of Oxytocin on the Superose 12 column has been discussed. The retention mechanism of Oxytocin on Superose 12 sames to be a mixed mode retention of hydrogen bond and ion interaction. And, established an acetic acid gradient elute method to accomplish the separation of oxytocin crude. (5) One step separation results of solid and liquid Oxytocin crude on Superose 12 shows that, salts of the crude palys an important rule in the separation process of Oxytocin on Superose 12. The crude should be desaulted when using Superose 12 as the separation and purification method. (6) Estabished the Polyamide desalting and Superose 12 purification method for the separation of oxytocin crude, under the optimized Superose 12 injection conditions, the the polyamide desalted samples could reach a purity of 95.4% and with the recovery