隆线溞线粒体基因组序列的测定与分析-植物学专业论文.docxVIP

隆线溞线粒体基因组序列的测定与分析Abstract 隆线溞线粒体基因组序列的测定与分析 Abstract 万方数据 万方数据 The Analysis of the Mitochondrial genome of Daphnia Carinata (Clasocera: Daphniidae) Li Zhang（botany） Abstract Daphnia carinata belongs to Daphniidae, Clasocera, Branchiapoda, Crustacea, Arthropoda, which plays important role in fresh-water ecosystem. The standard of their species identification has been contradictory, however, taking advantage of molecular biology methods might effectively revalue the taxonomy of different Daphnia animals, and reveal the true variations in the similar morphological group. This thesis aims to determine the sequence of its mitochondrial genome and analyze the genetic composition and structural characteristics, to provide a basis for the evolution analysis of Daphnia animals. In this research, we used Long-PCR and normal PCR methods to amplify the complete mitochondrial genome of Daphnia carinata, then determined and analyzed the PCR products. The full-length of mtDNA is 14 747 bp, in which the content of A, T, G, C is 33.8%, 36.5%, 14.4%, 15.3%, and the value of GC-Skew, AT-Skew were calculated as -0.009 and -0.027 respectively. By the analysis of sequence homology and structural characterization, we identified 36 genes, containing 13 protein coding genes, 21 tRNA and 2 rRNA genes, but the gene of tRNAIle, tRNAMet and control region can’t be sequenced. In 13 protein-coding genes, the start codons include ATA(ND5), ATG(COII, ATP6, COIII, ND4, CYTB, ND1, ND2), ATT(ND3, ND4L, ND6), GTG(ATP8), and a special start codon ACTA(COI), the stop codons include TAA(ATP6, ND4L, ND6, ND1), TAG(ATP8, COIII, ND3, CYTB), and four incomplete stop codons(COI, COII, ND5, ND4). The secondary structures of 21 predicted tRNA are typical cloverleaf, except the one of tRNASer(GCU) , which miss the dihydrouridine arm. Totally, there are III two U-C mismatches and one mismatch of U-U and A-A in all of the structure predictions. Among these 36 genes, 9 protein-coding genes(ND2, COI, COII, ATP8, ATP