颅内动脉粥样硬化性狭窄与纤维蛋白原β链基因多态性的相关性研究-内科学专业论文.docxVIP

PAGE PAGE 10 颅内动脉粥样硬化性狭窄与纤维蛋白原β链基因 多态性的相关性研究 中文摘要 目的：探讨颅内动脉粥样硬化性狭窄与纤维蛋白原 β 链基因 rs1800790、rs1800787 位点基因多态性之间的相关性。 方法：本研究采用病例对照研究，连续收集连云港市第二人民医院神经内科无血 缘关系的住院患者相关临床资料，同时检测血脂、血浆纤维蛋白原(fibrinogen, Fbg) 水平、C-反应蛋白(C-reactive protein, CRP)、同型半胱氨酸(homocysteine, Hcy)等实 验室指标。以是否存在颅内动脉粥样硬化性狭窄分为症状性颅内动脉狭窄组、小 血管病组、无症状性颅内动脉狭窄组和健康对照组。本研究采用 Taqman 实时荧光 定量 PCR 探针法进行 FGB 基因 rs1800790、rs1800787 位点基因分型。 结果：1. 本研究共入组患者 164 例，其中症状性颅内动脉狭窄组 81 例，小血管病 变组 43 例，无症状性颅内动脉狭窄组 15 例，健康对照组 25 例。2. 在传统卒中危 险因素中，CRP 升高与 IAS 呈显著相关(P=0.012)，而血浆 Fbg 水平和其他传统卒 中危险因素与 IAS 均无明显统计学相关性(P＞0.05)。3. rs1800787 T 等位基因频率 为 0.18，rs1800790 A 等位基因频率为 0.18。两位点各基因型频率和等位基因分布 在各组中差异无统计学意义。4. 血浆 Fbg 水平在 rs1800790 和 rs1800787 各基因型 之间无显著统计学差异(P 值均＞0.05)。5. FGB 基因 rs1800790 和 rs1800787 各等 位基因与 IAS 之间无显著相关性(P 值均＞0.05)。 结论：rs1800787、rs1800790 位点基因多态性与血浆 Fbg 水平和 IAS 均无显著相关 性。CRP 升高是 IAS 的独立危险因素。 关键词：颅内动脉狭窄；卒中；基因，多态性；纤维蛋白原；危险因素 Research in Relationship Between Intracranial Atherosclerotic Stenosis and Fibrinogen-beta Chain Genetics Polymorphisms ABSTRACT Objective: To investigate the relationship between intracranial atherosclerotic stenosis and genetics polymorphisms of rs1800787 and rs1800790 which are located in promoter area in FGB gene. Method: The research was a case-control study. Patients in Department of Neurology of NO.2 Peoples Hospital of Lianyungang were enrolled consistently from December, 2010 to February,2012. Basic characteristics,history and laboratory data including level of plasma fibrinogen were recorded. Patients enrolled were grouped into 4 groups,symptomatic intracranial atherosclerotic stenosis(IAS) group (SIAS), ischemic stroke caused by small vessel disease group(SVD), asymptomatic IAS group(aSIAS) and health control(HC).We used Taqman real-time polymerase chain reaction(PCR) for genotyping. Results: 1.A total of 164 patients were enrolled,81 for SIAS group,43 for SVD group,15 for aSIAS group and 25 for HC. 2.As for classical stroke risk factors, level of CRP was in relation to IAS independently(P=0.012