鲤鱼鱼皮胶原蛋白交联物的制备研究-水产品加工及贮藏工程专业论文.docxVIP

摘要 摘要 Ab Abstract I I II II 摘 要 本文以鲤鱼的鱼皮为原料，研究了鲤鱼皮提取胶原蛋白的条件、胶原蛋白交联物的制 备工艺以及交联物的部分性质。主要过程及结果如下：。 1、确定了鲤鱼皮胶原蛋白的工艺条件。在 2.0 %的乙酸，鱼皮（W）和乙酸（V） 的料液比 1:40，浸泡时间 180 min 的条件下，鱼皮胶原蛋白提取率为 60.0%。氨 基酸组成、SDS电泳图谱和傅立叶红外光谱的比对分析表明，所提胶原 蛋白是 I 型胶原蛋白，其最大吸收波长为 234 nm。 2、研究了戊二醛作为交联剂的胶原蛋白与壳聚糖交联物的制备工艺。正交试验优化 的交联反应条件为：胶原蛋白与壳聚糖构成比例为 6:4，胶原蛋白和壳聚糖总浓 度为 0.8%，戊二醛浓度为 2.0%，交联物的吸水倍数为 48.4 倍，抗张强度为 100.5 N/mm2。凝血指数 23.0，冻干后呈海绵状固体。 3、分析了交联产物的理化性质。测得上述所制备的交联度为 33.8 %。交联物表观 密度为 0.0125 g/mm3，孔隙率为 78.0 %。 4、通过吸水倍数、抗张强度、凝血指数的对比发现，本交联物的均比纱布、未加戊 二醛的胶原蛋白-壳聚糖交联物、市售的明胶止血海绵和胶原蛋白四种物质对比 发现其优异，反映出鲤鱼皮胶原蛋白可以作为止血交联物的原料。 关键词：鲤鱼皮；胶原蛋白；壳聚糖；戊二醛；交联； Abstract In this paper, carp skin was used as raw material to study the conditions of extracting collagen from carp skin, the preparation process of cross-linked collagen and some properties of cross-linked product. The main process and results are as follows: The process conditions of carp skin collagen were determined. With acetic acid 2.0%, the solid-liquid ratio of skin (W) and acetic acid (V) 1:40 and soaking time 180 min, the extraction rate of skin collagen was 60.0%. Amino acid composition, SDSpattern and fourier transform infrared spectroscopy showed that the collagen was I type collagen with the maximum absorption wavelength of 234 nm. The preparation process of collagen-chitosan cross-linked products using glutaraldehyde as a crosslinking agent was studied. The crosslinking reaction conditions determined by orthogonal experiment were: the composition ratio of collagen and chitosan 6:4, the total concentration of collagen and chitosan 0.8%, the concentration of glutaraldehyde 2.0% , the absorbent multiples of cross-linked product was 48.4 and the tensile strength 100.5 N/mm2. The coagulation index was 23.0 and cross-linked product was spongy solid after lyophilization. The physicochemical properties of cross-linked product were analysed. The degree of crosslinking of cross-linked product was 33.8% with apparent density 0.0125 g / mm3 and porosity 78.0%. By contrast with