清瘟败毒合剂解热抗炎抗菌作用的实验研究.docVIP

清瘟败毒合剂解热抗炎抗菌作用的实验研究 王晓1 杨艳平2 (1汉中市第二药材公司723000； 2江西中医学院 江西南昌330004) 【摘要】目的：探讨清瘟败毒合剂的解热抗炎抗菌作用。方法：采用乙4? 二硝基苯酚致大鼠发热法研究清瘟败毒合剂的解热作用，采用二甲苯致小鼠耳肿 胀法和蛋清致大鼠足肿胀法研究清瘟败毒合剂的抗炎作用。采用琼脂稀释法测定 清瘟败毒合剂对4种菌的最低抑菌浓度(MIC)o结果：与空白对照组比较，清瘟 败毒合剂对2,4 ■二硝基苯酚引起的大鼠体温升高具有良好的解热作用(Plt;0.05)； 并能显著抑制二甲苯致小鼠耳肿胀及蛋清致大鼠足跖肿胀的炎症反应(Plt;0.05)。 金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白念珠菌对清瘟败毒合剂均敏感。结论： 清瘟败毒合剂具有解热、抗炎作用，同时对金黄色葡萄球菌、大肠杆菌、绿脓杆 菌、白念珠菌均具有体外抑菌作用。 【关键词】清瘟败毒合剂解热抗炎抗菌 【中图分类号】R96 【文献标识码】A 【文章编号】2095-1752 (2014) 13-0110-02 Studies on antipyretic,anti-iinflammatory and antibacterial effects of Qingwenbaidu Mixture IWangXiao, Yang Yanping2 (lGrassland,the second medicinal materials company 723000; 2Jiangxi University of Traditional Chinese Medicine, Nanchang 330004) 【Abstract 】Objective: To study the antipyretic ‘anti-inflammatory and antibacterial effects of Qingwenbaidu Mixture. Methods: The antipyretic activity of Qingwenbaidu Mixture was studied in rats with 2,4-dinitrophenol’and the inflammatory activity was studied in rats with foot edema induced by plain white,mice with ear edema induced by the xylene. Agar dilution method were used to measure of minimum in hibitory concen trati on of four kinds of bacteria .Results: Compared with the control group,Qingwenbaidu Mixture showed significant antipyretic activity in rats with high temperature induced by 2,4-dinitrophenol (Plt;O. 05) ,and showed significant anti- inflammatory activity in rats with foot edema induced by plain white,mice with ear edema induced by the xylene ( Plt;0. 05). Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans had sensitivity for Qingwenbaidu Mixture.C on clusi on: Qin gwe nbaidu Mixture has no table effects of a ntipyretic and anti-inflammatory. The results showed that Qingwenbaidu Mixture have antibacterial effect on S. aureus, E. coli, Pseudomonas aeruginosa and Candida albicans in vitro. 【Key words] Qingwenbaidu Mixture Antipyretic Anti-inflammatory An tibacterial 清瘟败毒合剂由生石膏、生地、知母、水牛角、黄连、黃苓、梔子等组成, 是在古代名方清瘟败毒饮基础上加减化裁，釆用现代制剂技术提