微生物发酵降解植物甾醇侧链生产17_酮甾体研究探究进展资料精.pdfVIP

8 5 Vo.l 8 No. 5 2010 9 Ch inese Journal of B ioprocess Engineering Sep. 2010 do:i 10. 39 9/ .j issn. 1 72- 3 78. 2010. 05. 015 17 1 1 1 2 2 杨顺楷 , 杨亚力 , 吴中柳 , 范林萍, 李小刚 ( 1. 中国科学院 成都生物研究所, 成都 10041; 2. 兰州大学 生命科学学院, 兰州 730000) : 微生物发酵降解植物甾醇侧链, 生产雄甾4烯3, 17二酮(AD), 雄甾1, 4二烯3, 17二酮(ADD), 和 9羟 基AD甾体药物中间体的工业生物技术对改 制造甾体激素药物半合成原料薯蓣皂素短缺的现状, 实现甾体激素 药物半合成原料多元化, 合理利用我国甾体植物资源具有重要意义重点评述了近期微生物法断植物甾醇侧链制 ADADD和 9羟基AD的研究现状, 内容包括: 1) 微生物菌种选育; 2)菌种相关的细胞生理, 酶学性质和生物催化 过程; 3)相关酶的细胞定位及生物反应器; 4)发酵工艺选择和甾醇原料的合理利用 : 生物降解; 微生物发酵; 植物甾醇 : TQ4 42 : A : 1 72- 3 78( 2010) 05- 00 9- 09 M icrobial ferm entation of phytosterol sidechain cleaved for production of 17keto steroids 1 1 1 2 2 YANG Shunkai, YANG Yali, WU Zhongliu , FAN L inp ing , LIX iaogang ( 1. Chengdu Institute of B iology, Ch inese Academy of Sciences, Chengdu 1004 1, Ch ina; 2. School of Life Sciences, Lanzhou Un iversity, Lanzhou 730000, China) Abstract: The study on the m icrob ial degration of phytosterol sidechain and the development of industral b iotechnology for production of 17keto steroids, i. e. , androst4ene3, 17dione ( AD), androst1, 4 diene3, 17dione, and 9hydroxyAD, are of sign ificance to rep lace partially diosgen in as its shortage w ith them, and to perform mu ltiple sources as one k ind of raw materals of sem isynthetic production in steroidpharmaceutical industry, and to put plant steroid resources to rational use in China for today. Th is paper review edm icrob ial organism for the breeding and selection mutant, the strain selection of related