脂立方相技术体系在膜蛋白结构与功能研究中的应用进展.pdf

中国细胞生物学学报 Chinese Journal of Cell Biology 2015, 37(7): 925–935 DOI: 10.11844/cj cb.2015.07.9002 特约综述 本实验室的研究兴趣集中于膜蛋白结构生物学和发展与其紧密关联的脂 立方相技术体系。对脂立方相结晶技术体系进行简化和优化, 建立脂立方 相用于膜蛋白生化分析的新方法, 并应用于与人类健康相关膜蛋白质的结 构和功能研究, 揭示功能机制, 为理解和治疗相关疾病提供理论基础。 http :///ePI.asp?id= 157 脂立方相技术体系在膜蛋白结构与 功能研究中的应用进展 姚贺帮 蔡洪敏 李典范* ( 中国科学院上海生命科学研究院生物化学与细胞生物学研究所, 国家蛋白质科学中心, 分子生物学国家重点实验室, 上海2012 10) 摘要 脂立方相是包含连续脂双层和水通道的材料。其作为跨膜蛋白质结晶的有效介质, 是一些重要和高难度膜蛋白结构解析的关键。因此, 脂立方相技术体系越来越受到结构生物学家 的重视。该文就脂立方相的特性及其结晶相关技术的最新进展作一简要阐述, 并介绍该技术体系 在膜蛋白生化分析与体外复性研究中的应用现状。 关键词 膜蛋白; 脂立方相; 结晶; 酶学分析; 体外复性 Lipid Cubic Phase Technology for Membrane Protein Structural and Functional Research Yao Hebang, Cai Hongmin, Li Dianfan* (National Center for Protein Sciences (Shanghai), State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 201210, China) Abstract Composed of a continuous bilayer and two continuous water channels, lipid cubic phase has been proven as a promising method for membrane protein crystallization. Since its introduction into the field 19 years ago, it has contributed to the structure determination for many challenging membrane proteins, some of which has puzzled structure biologists for decades. As such it has become increasingly popular. This review aims to give a ba- sic description to the method and to briefly summarize the advances for this technology. The application of the lipid cubic phase in membrane protein functional assays and refolding is also described. Ke