HERG基因新突变与家族性QT延长综合征.pdf

from DNAisobtainedblood 2．Genomic anticoagulation samples．The was withthe of and blood doled K，SDS，chloroform reagentsprotease alcoholtoextract and DNA．Moreover,the anhydrous genomic productpurlty ofextractionwere evaluated by initially spectrophotography． chain wet-e reaction(PCR)：Pfilner 3．Polymerase pairs designed totheeXOll of5related according sequences genes KVLQTl，HERG, including andSCN5A．The were SoRware Mink,MIRP primerpairs parUydesignedby of the Primer．Premier5．0．Allof primer were in bank． pairs comparedgone Different andtermswere to the systems employed amplifytarget sequences， whichwere and screened preliminarily quantifiedbyagarosegel electrophoresis． chain strand confommtion PER- 4．Polymerasereaction-single polymorphism tothecharacterofthePCR SSCP)：According products，we employed suitable polyacrylamidegelelectrophoresis(PAGE)in