发状念珠藻与集胞藻 小球藻的营养成走分及在盐胁迫下rubisco基因表达的比较.docxVIP

和EPSX中还存在仅．糖苷键。分子量测定结果表明EPSF为低分子量多聚糖 和EPSX中还存在仅．糖苷键。分子量测定结果表明EPSF为低分子量多聚糖 而EPSJ和EPSX为高分子量多聚糖且组分相对均一，分子量分别为2．5 kDa、 705．5 kDa和105．1 kDa。氧自由基清除能力测定结果表明：EPSF表现出更 强的氧自由基清除能力，为78．96 gmol·Trolox g～，EPSJ次之为44．29 gmol·Trolox g～，EPSX最小为24．65 gmol·Trolox g一。 (3)集胞藻能适应浓度为0-0．7 mol／L的NaCl胁迫，小球藻能够适应 浓度为O～O．3 mol／L的Nacl胁迫，两种藻盐胁迫时的生长速率均低于正常培 养。在盐胁迫时，发状念珠藻细胞形态发生显著变化，细胞变黄，链式结构 逐渐瓦解并分散成单个营养细胞，集胞藻和小球藻的细胞形态未出现明显变 化，分裂细胞的个数随盐胁迫时间的延长而逐渐减少。 (4)正常培养及盐胁迫(O．3 mol／L)培养条件下，发状念珠藻的生长 速率及Rubisco活力都低于集胞藻和小球藻。正常培养时，发状念珠藻 Rubisco基因的表达量稳定在O．35以内，但其最大表达量始终低于小球藻； 盐胁迫培养(O．3 mol／L)时，发状念珠藻Rubisco基因的表达量稳定在 O．12--0．48以内，小球藻中Rubisco基因的表达量变化较大，培养后期接近于 O，集胞藻Rubisco基因的表达量虽然较低(最大为0．34)，但结合其生长 曲线及酶活性分析得出，集胞藻Rubisco的羧化活性要高于发状念珠藻，在 盐胁迫条件下能够保持快速生长。 关键词：发状念珠藻，集胞藻，小球藻，营养成分，胞外多糖，1，5一二磷酸 核酮糖酶 万方数据 Comparison Comparison of Nutrients Between NostocflageUiforme、 Synechocystiss and Chlorella，and Rubisco Gene Expression Under Salt Stress ABSTRACT As an important food-type nitrogen fixing cyanobacteria，Nostoc flagelliforme also known as“facai’’，mainly distributed in country—semi—arid regions．Nostoc flagelliforme has an extremely hi曲nutritional value and is rich in amino acids，carbohydrates，protein and Ca,Mg，K，Na，etc．Besides， extracellular polysaccharide(EPS)separated from Nostocflagelliforme is also an active substance that has great potential for natural medicine development． However．because of the slow pace of natural reproduction and irrational exploitation．Nostoc flagelliforme is on the verge of extinction now．This high demand and low supply of Nostoc flagelliforme and its EPS is the main challenge and roadblock of its commercialization．So how to improve the growth efficiency of Nostoc flagelliforme is a maj or concern．It has been found that the yield and activity of Nostoc flagelliforme EPS was higher under salt—stressed culture than normal conditions． Based on this findings，in this paper， we compared main nutrients among Nostoc flagelliforme，Synechocystiss and Chlorella．And then isolated and purified their EPS，co