二穗短柄草对低钙胁迫的响应及止其谷氨酸受体家族基因鉴定和功能分析-生物化学与分子生物学专业毕业论文.docxVIP

动物离子型谷氨酸受体(iGLuRs)和拟南芥AtGLRs结构类似。二穗 动物离子型谷氨酸受体(iGLuRs)和拟南芥AtGLRs结构类似。二穗 短柄草谷氨酸受体基因(BdGLRs)可分为三个亚类。同时我们对 BdGLRs基因表达的组织特异性进行了半定量RT-PCR分析，发现绝大 多数BdGLRs在根、茎、叶中表达，但不同的BdGLRs在各组织表达 量有所不同。在二穗短柄草根细胞，Glu可以诱导Ca2+的内流和调节 根结构，说明在二穗短柄草中也存在对Glu的响应这一机制。 关键词：二穗短柄草；低钙胁迫；表型；谷氨酸受体 万方数据 内蒙山大学硕士学位论文THE 内蒙山大学硕士学位论文 THE RESPONSE oF占R／重C日卯D_W聊脚2■C日y()}ⅣIN Low CALCIUM STRESS AND lTS GLUTAMATE RECEPTOR FAMILY GENE IDENTIFICATION AND FUNCTION ANALYSIS ABSTRACT Ca2+is one of the most important nutrient elements in plants．But the calcium that plants can use has declined in the soil and water year by year, as well as the animals．After Brachypodium distachyon genome was sequenced in 201 1，the new mode 6f gramineous plant was begen to be study depthly．Brachypodium distachyon Bd2 1 was treated with low calcium to analyze the phenotype and absorption of elements，and the microstructure change．At the same time，changes of gene expression were analyzed in the stress of low calcium．Expression profile chip analysis showed AtGLR2．8 gene which was annoted in Arabidopsis expression rose observably．Glutamate(Glu)is one of primary neurotransmitters for neurons communication mediated by glutamate receptor(GLRs)on the membrane．Glutamate receptors in mammals are divided into two families： ionic glutamate receptor(iGLuRs)and metabolism of glutamate receptors (mGLuRs)．Physiological，pharmacology and genetic evidences indicate that．Arabidopsis and Rice have the similar signal transduction mechanism 第3页 万方数据 内蒙古大学硕士学位论文as 内蒙古大学硕士学位论文 as that in mammals．In this study，19 glutamate receptor genes were found in Brachypodium distachyon genome through bioinformatics analysis． These receptors had conservative amino acid binding sites and membrane structure domain，and were similar with the ionic glutamate receptor (iGLuRs)and AtGLRs in Arabidopsis thaliana．Glutamate receptors in Brachypodium distachyon(BdGLRs)can be divided into three classes and their expression showed issue specificity．Glu could induce Ca2+influx in the r