依托咪酯对大鼠海马CA1区兴奋性突触的影响部位探讨及对兴奋性.doc

依托咪酯对大鼠海马CA1区兴奋性突触的影响部位探讨及对文中并未有相关指标并未探讨依托咪酯对兴奋性突触的部位的研究兴奋性突触后电流成分的影响 文中并未有相关指标并未探讨依托咪酯对兴奋性突触的部位的研究 于婵娟,刘亚华,侯彦深，史春慧，蒋 晖 （830011 乌鲁木齐，新疆医科大学附属肿瘤医院麻醉科） [摘要] 目的 探讨依托咪酯对大鼠海马CA1区兴奋性突触影响的可能部位。在依托咪酯的影响下，大鼠海马CA1区兴奋性突触后电流（excitatory postsynaptic currents，EPSC）的成分变化。方法 将制备好的海马脑片分为两组，分别以60μl脂肪乳剂或依托咪酯（相当于10μmol/L）孵育海马脑片40min，。（1）在Schaeffer 侧支/联合纤维连续给予配对刺激P1、P2，P1、P2（间隔（interpulse interval，IPI）为50～80ms），每一时间间隔下给予10次配对刺激，在CA1区锥体神经元记录不同时间间隔下配对刺激诱发的两次EPSC的比率，即EPSC2/EPSC1。膜钳制电压为-70mV。（2）在Schaeffer 侧支/联合纤维给予单刺激，同时改变CA1区锥体神经元细胞膜钳制电压（-100～+40 mV，变化幅度为20mV），制作电流-电压（I-V）曲线方法描述过于繁琐。结果 （1）在膜钳制电压为-70mV条件下，10μmol/L脂肪乳剂组EPSC2/EPSC1值和10μmol/L依托咪酯组EPSC2/EPSC1值比较，差异无显著性（P0.05）。（2）10μmol/L依托咪酯脂肪乳剂降低了反转电位，电位由-53mV左右降为-60mV左右，使I-V曲线左移。结果描述过于简单结论 本浓度的依托咪酯对突触前膜的影响较小，其对构成EPSC的离子成分发生了变化，使外向电流成分增加，可能主要是氯离子。因未做膜片钳无法确定外向电流成分，不宜在结论中推测可能是氯离子 方法描述过于繁琐 结果描述过于简单 因未做膜片钳无法确定外向电流成分，不宜在结论中推测可能是氯离子 [关键词] 兴奋性突触后电流；电流-电压曲线；依托咪酯；海马 [中图法分类号] [文献标志码] A Effects of etomidate on spontanous excitatory postsynaptic current in hippocampal CA1 region of rats Yu Chanjun, Liu Yahua, Hou Yanshen,Shi Chunhui,Jiang Hui(Department of Anesthesiology, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China) [Abstract] Objective By whole-cell voltage-clamp technique, research the effect site of etomidate emulsion on excitatory synaptic of hippocampal CA1.And analysis the effect of etomidate emulsion to the I-V curve of excitatory postsynaptic currents of hippocampus CA1,discuss the change of EPSC .Methods The 20 hippocampal slices were evenly divided into two groups. They were emulsion group, etomidate of 10μmol group. (1)pairing stimulation was used to observe the effect of 10μmol/L etomidate emulsion on the ratio of EPSC2/EPSC1 of hippocampal CA1.(2) Single stimulation was used to obtain the effect of 10μmol/L etomidate emulsion on the curve of current and voltage (I-V).Results (1)At a holding potential of -70mV,there was no difference of 10μmol/L etomidate