B2013acterial degradation of pyrene in minimal salt medium mediated英文学习资料 .pdfVIP

Bioresource Technology 133 (2013) 293–300 Contents lists available at SciVerse ScienceDirect Bioresource Technology journal homepage: www.elsevi /locate/biortech Bacterial degradation of pyrene in minimal salt medium mediated by catechol dioxygenases: Enzyme purification and molecular size determination S.N. Singh a,⇑, Babita Kumari a, Santosh Kumar Upadhyay b, Shweta Mishra a, Dileep Kumar c a Environmental Science Division, CSIR-National Botanical Research Institute, Lucknow 226001, UP, India b Plant Molecular Biology and Genetic Engineering Division, CSIR-National Botanical Research Institute, Lucknow 226001, UP, India c Microbial Biotechnology, CSIR-North-East Institute of Science and Technology, Jorhat, India h i g h l i g h t s Microbial degradation of pyrene in MSM. Involvement of catechol 1,2 dioxygenase and catechol 2,3 dioxygenase in pyrene degradation. Purification and molecular size determination of catechol 1,2 dioxygensae. a r t i c l e i n f o a b s t r a c t Article history: In vitro degradation of pyrene was studied in MSM by three bacterial strains individually, designated as Received 12 November 2012 BP10, NJ2 and P2. Among these strains, NJ2 was the highest degrader (60%) of pyrene, followed by BP10 Received in revised form 11 January 2013 (44%) and the least was P2 (42%) in MSM with pyrene (50 lg ml1) in 8 days. During pyrene degradation, Accepted 12 January 2013 catechol 1,2 dioxygenase (C12O) activity was induced by 13 folds in BP10 and 17 folds in P2 as compared Available online 4 February 2013