过敏性紫癜发病中树突状细胞与T辅助细胞分化失衡的关系.docVIP

过敏性紫癜发病中树突状细胞与T辅助细胞分化失衡的关系 陶红，李秋，王莉，李翠萃，阳海平，张高福（400014 重庆 重庆医科大学附属儿童医院肾脏免疫科） 【摘要】目的 探讨树突状细胞(dendritic cells，DC)调控信号的异常与过敏性紫癜(Henoch-Schonlein purpura，HSP)发病中T辅助细胞分化失衡的关系。方法 分别采用散射比浊法、ELISA和流式细胞技术，检测HSP患儿血浆中免疫球蛋白、血浆IL-4 、INF-γ和DC培养上清IL-12、IL-10水平及DC表面CD86、CD80、CD83、HLA-DR、CD-209的表达情况。结果 与对照组比较，HSP组患儿：血浆免疫球蛋白IgA及IgE水平升高；血浆中TH2类细胞因子IL-4升高，TH1类细胞因子INF-γ降低；DC培养上清中IL-10升高，IL-12降低；DC表达CD86、CD83、HLA-DR水平升高，表达CD80水平降低，P＜0.05。CD-209在两组之间差异无统计学意义，P＞0.05。结论 DC的抗原特异性信号、协同刺激信号和分化或极化信号三种调控信号的异常，与HSP患儿TH1/TH2失衡、免疫球蛋白合成异常密切相关。 【关键词】树突状细胞； T淋巴细胞；过敏性紫癜 The relation of dendritic cell to the imbalance differentiation of T helper cell in pathogenesis of Henoch-Schonlein purpura TAO Hong, LI Qiu, WANG Li, LI Cuicui, YANG Haiping, ZHANG Gaofu (Department of Kindey immunity ,the Children’s Hospital of Chongqing Medical University , Chongqing 400014,China) 【Abstract】Objective Discussion the relation between the abnormal regulation signals of dendritic cells(DC) and the dysimmunity of pathogenesis of Henoch-Schonlein purpura （HSP）. Methods Immunoglobulin Levels were detected by nephelometry assay in children with HSP . Inflammatory cytokines IL-4, INF-γ in blood plasma and IL-12, IL-10 in the supernatants of cultured DC were detected by ELISA. The count of CD86, CD80, CD83, HLA-DR, CD-209 expressed on DC were detected by cell flow cytometry. Results HSP group showed: The levels of plasma IgA and IgE were higher than the control group, IL-12 levels in the supernatants of cultured DC and INF-γ levels in the plasma were lower than the control group,IL-10 levels in the supernatants of cultured DC and IL-4 levels in the plasma were significantly increased than the control group. CD86, CD83, HLA-DR expressed on DC were significantly higher and CD80 was significantly lower than the control group, P＜0.05. CD-209 of Two groups had no statistical significance, P0.05. Conciusion The anomalies of antigenspecific signals, costimulatory signals and differentiation or polarization signal of DC mig