不同炮制方法对延胡索中有效成分含量的影响.docVIP

不同炮制方法对延胡索中有效成分含量的影响来源： 中国论文下载中心????[ 11-05-04 16:59:00 ]????编辑：studa20 ????? ?????????????? 作者：曹柳,窦志英,王萍,田永亮 【摘要】? 目的 比较不同炮制方法对延胡索中3种有效成分含量的影响。方法 将鲜延胡索、水煮延胡索和市售的延胡索药材分别经醋煮、醋炙和酒炙后,分别采用水超声法和甲醇热回流法进行提取,采用反相高效液相色谱法测定3种有效成分的含量。色谱柱为Agilent TC-C18(250 mm×4.6 mm,5 μm),流动相：乙腈-0.1%磷酸溶液(三乙胺调pH 5.3) 为28∶72,流速：1 mL/min,柱温：35 ℃, 【关键词】? 延胡索；原阿片碱；去氢紫堇碱；延胡索乙素；高效液相色谱法；炮制 ??? Abstract：Objective To explore the effect of different processes on the contents of active ingredients in Rhizoma Corydalis. Methods Processes of vinegar-boiling, vinegar-frying and alcohol-frying were used to fresh corydalis, boiled corydalis and purchased corydalis. Then the method of extraction by ultrasound with water and by reflux with methanol were used to extract the sample. The contents of Protopine, Tetrahydropalmatine and Dehydrocorydaline in corydalis was determined by RP-HPLC. Agilent TC-C18 (25 mm×4.6 mm, 5 μm) column was used at 35 ℃, with acetonitrile-0.1% phosphonic acid (pH 5.3 with triethylamine) (28∶72) as mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 280 nm. Results In the sample extracted by water, the contents of active ingredients in processed products were higher than in corydalis. And in the sample extracted ??? Key words：Corydalis；Protopine；Dehydrocorydaline；Tetrahydropalmatine；HPLC；process???? 延胡索为罂粟科植物延胡索Corydalis yanhusuo W.T.Wang的干燥块茎,具有活血、利气、止痛功效,临床用于 治疗胸胁、脘腹疼痛、经闭痛经、产后瘀阻、跌扑肿痛等症[1],主产于浙江。延胡索中主要有效成分为生物碱,生物碱在药材中多数以结合态的生物碱盐存在,少数碱性极弱的以游离态形式存在。延胡索的药理作用表现为镇痛、催眠、降低冠脉阻力及增加血流量等,另外亦对心血管系统有较好的药理活性。延胡索多炮制后入药,主要炮制方法为醋煮、醋炙和酒炙。本试验采用水超声提取法和甲醇加热回流提取法分别提取总生物碱盐和总生物碱,通过高效液相色谱法比较了不同炮制方法对延胡索中延胡索乙素、原阿片碱和去氢紫堇碱含量的影响,为优化延胡索炮制工艺和阐明炮制机理奠定基础。 　　1? 仪器与试药 ??? 烘箱DHG78-1(湖北省黄石市医疗器械厂)；日本岛津HPLC系统(SCL-10AVP HPLC Pump；SPD-ML0AVP二级管阵列检测器)；自动进样系统；超声波清洗器AS20500AT(天津奥特赛恩斯仪器有限公司)。甲醇、乙腈为色谱纯(天津市协和昊鹏色谱科技有限公司)；其余试剂均为分析纯；水为超纯水。延胡索乙素(批号0726-200409, 中国药品生物制品检定所)；去氢紫堇碱(批号043-27611,日本和光纯药 工业株式会社)；原阿片碱(批号0853-200201,中国药品生物制品检定所)。 ??? 鲜延胡索药材产自浙江东阳；市售延胡索购于天津中新药业集团股份有限公司药材分公司,产自浙江。经本校中药鉴定教研室马琳教授鉴定为